UniProt Accession

 HXKB_YEAST; P04807; D6VV82; Q05838 

Genbank Protein ID

 X03483; M11181; M14411; X94357; Z72775; X67787; BK006941 

Genbank Nucleotide ID

 CAA27203.1; AAA34697.1; AAA34699.1; CAA64134.1; CAA96973.1; CAA48003.1; DAA07866.1 

Protein Name

 Hexokinase-2 

Protein Synonyms/Alias

 Hexokinase PII; Hexokinase-B 

Gene Name

 HXK2 

Gene Synonyms/Alias

 HEX1; HKB; YGL253W; NRB486 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
21	GSMADVPKELMQQIE	acetylation	[1]
54	HFISELEKGLSKKGG	acetylation	[1]
98	NLRVVLVKLGGDRTF	acetylation	[1]
111	TFDTTQSKYRLPDAM	acetylation	[1]
111	TFDTTQSKYRLPDAM	ubiquitination	[2]
194	DVVPMLQKQITKRNI	acetylation	[1]
284	HVVLPRTKYDITIDE	acetylation	[1, 3]
329	YKQGFIFKNQDLSKF	acetylation	[1]
335	FKNQDLSKFDKPFVM	acetylation	[1]
338	QDLSKFDKPFVMDTS	acetylation	[1]
338	QDLSKFDKPFVMDTS	ubiquitination	[2]
406	GIAAICQKRGYKTGH	ubiquitination	[2]
410	ICQKRGYKTGHIAAD	acetylation	[1]
430	RYPGFKEKAANALKD	ubiquitination	[2]
473	VIAALAQKRIAEGKS	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 

Functional Description

 Main glucose phosphorylating enzyme. May play a regulatory role in both induction and repression of gene expression by glucose. 

Sequence Annotation

 NP_BIND 86 91 ATP (By similarity).
 NP_BIND 307 308 ATP (By similarity).
 NP_BIND 344 348 ATP (By similarity).
 NP_BIND 419 423 ATP (By similarity).
 REGION 175 176 Substrate binding (By similarity).
 REGION 210 211 Substrate binding (By similarity).
 BINDING 111 111 ATP (Potential).
 BINDING 158 158 Substrate; via carbonyl oxygen (By
 BINDING 237 237 Substrate (By similarity).
 BINDING 269 269 Substrate (By similarity).
 BINDING 302 302 Substrate (By similarity).
 MOD_RES 15 15 Phosphoserine.
 MOD_RES 38 38 Phosphothreonine.
 MOD_RES 158 158 Phosphoserine.
 MOD_RES 245 245 Phosphoserine.  

Keyword

 3D-structure; Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 486 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004396; F:hexokinase activity; IDA:SGD.
 GO:0032445; P:fructose import; IGI:SGD.
 GO:0006000; P:fructose metabolic process; IMP:SGD.
 GO:0046323; P:glucose import; IGI:SGD.
 GO:0006096; P:glycolysis; IDA:SGD.
 GO:0006013; P:mannose metabolic process; IDA:SGD.
 GO:0008361; P:regulation of cell size; IMP:SGD.
 GO:0046015; P:regulation of transcription by glucose; IDA:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD. 

Interpro

 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 

Pfam

 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 

SMART

  

PROSITE

 PS00378; HEXOKINASES 

PRINTS

 PR00475; HEXOKINASE.