CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014367
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable ATP-dependent RNA helicase DDX5 
Protein Synonyms/Alias
 DEAD box RNA helicase DEAD1; mDEAD1; DEAD box protein 5; RNA helicase p68 
Gene Name
 Ddx5 
Gene Synonyms/Alias
 Tnz2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
53WNLDELPKFEKNFYQsumoylation[1, 2]
236IDFLECGKTNLRRTTacetylation[3]
340MEEIMSEKENKTIVFubiquitination[4]
437GRTARSTKTGTAYTFubiquitination[4]
470ANQAINPKLLQLVEDacetylation[5, 6]
470ANQAINPKLLQLVEDubiquitination[4]
523RGYSNLLKRDFGAKTubiquitination[4]
Reference
 [1] SUMO modification of the DEAD box protein p68 modulates its transcriptional activity and promotes its interaction with HDAC1.
 Jacobs AM, Nicol SM, Hislop RG, Jaffray EG, Hay RT, Fuller-Pace FV.
 Oncogene. 2007 Aug 30;26(40):5866-76. [PMID: 17369852]
 [2] DEAD-box RNA helicases as transcription cofactors.
 Fuller-Pace FV, Nicol SM.
 Methods Enzymol. 2012;511:347-67. [PMID: 22713328]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. 
Sequence Annotation
 DOMAIN 125 300 Helicase ATP-binding.
 DOMAIN 328 475 Helicase C-terminal.
 NP_BIND 114 116 ATP (By similarity).
 NP_BIND 138 145 ATP (By similarity).
 REGION 477 614 Transactivation domain (By similarity).
 MOTIF 94 122 Q motif.
 MOTIF 248 251 DEAD box.
 BINDING 121 121 ATP (By similarity).
 MOD_RES 32 32 N6-acetyllysine (By similarity).
 MOD_RES 33 33 N6-acetyllysine (By similarity).
 MOD_RES 40 40 N6-acetyllysine (By similarity).
 MOD_RES 297 297 Phosphotyrosine (By similarity).
 MOD_RES 480 480 Phosphoserine.
 MOD_RES 502 502 Omega-N-methylated arginine (By
 CROSSLNK 53 53 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; ATP-binding; Complete proteome; Helicase; Hydrolase; Isopeptide bond; Methylation; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 614 AA 
Protein Sequence
MSSYSSDRDR GRDRGFGAPR FGGSRTGPLS GKKFGNPGEK LVKKKWNLDE LPKFEKNFYQ 60
EHPDLARRTA QEVDTYRRSK EITVRGHNCP KPVLNFYEAN FPANVMDVIA RQNFTEPTAI 120
QAQGWPVALS GLDMVGVAQT GSGKTLSYLL PAIVHINHQP FLERGDGPIC LVLAPTRELA 180
QQVQQVAAEY CRACRLKSTC IYGGAPKGPQ IRDLERGVEI CIATPGRLID FLECGKTNLR 240
RTTYLVLDEA DRMLDMGFEP QIRKIVDQIR PDRQTLMWSA TWPKEVRQLA EDFLKDYIHI 300
NIGALELSAN HNILQIVDVC HDVEKDEKLI RLMEEIMSEK ENKTIVFVET KRRCDELTRK 360
MRRDGWPAMG IHGDKSQQER DWVLNEFKHG KAPILIATDV ASRGLDVEDV KFVINYDYPN 420
SSEDYIHRIG RTARSTKTGT AYTFFTPNNI KQVSDLISVL REANQAINPK LLQLVEDRGS 480
GRSRGRGGMK DDRRDRYSAG KRGGFNTFRD RENYDRGYSN LLKRDFGAKT QNGVYSAANY 540
TNGSFGSNFV SAGIQTSFRT GNPTGTYQNG YDSTQQYGSN VANMHNGMNQ QAYAYPLPQA 600
APMIGYPMPT GYSQ 614 
Gene Ontology
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0030529; C:ribonucleoprotein complex; ISO:MGI.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0050681; F:androgen receptor binding; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0030331; F:estrogen receptor binding; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003724; F:RNA helicase activity; ISS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; ISS:UniProtKB.
 GO:0007623; P:circadian rhythm; IMP:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0072332; P:intrinsic apoptotic signaling pathway by p53 class mediator; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0043517; P:positive regulation of DNA damage response, signal transduction by p53 class mediator; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
 GO:0060765; P:regulation of androgen receptor signaling pathway; ISS:UniProtKB.
 GO:0045667; P:regulation of osteoblast differentiation; IMP:UniProtKB.
 GO:2001014; P:regulation of skeletal muscle cell differentiation; IMP:UniProtKB.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR012587; P68HR.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C
 PF08061; P68HR 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS