| Tag | Content |
|---|
| CPLM ID | CPLM-019716 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 |
Protein Synonyms/Alias | AGAP-2; Centaurin-gamma-1; Cnt-g1; GTP-binding and GTPase-activating protein 2; GGAP2; Phosphatidylinositol 3-kinase enhancer; PIKE |
Gene Name | AGAP2 |
Gene Synonyms/Alias | CENTG1; KIAA0167 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 1037 | RESWIRAKYEQLLFL | ubiquitination | [1] |
|
Reference | [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA. Mol Cell Proteomics. 2012 May;11(5):148-59. [ PMID: 22505724] |
Functional Description | GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. |
Sequence Annotation | DOMAIN 676 910 PH.
DOMAIN 931 1051 Arf-GAP.
REPEAT 1090 1119 ANK 1.
REPEAT 1123 1152 ANK 2.
NP_BIND 413 420 GTP (Potential).
NP_BIND 457 461 GTP (Potential).
NP_BIND 515 518 GTP (Potential).
ZN_FING 946 969 C4-type.
REGION 1 23 Interaction with EPB41L1 (By similarity).
REGION 180 225 Interactions with HOMER1 and NF2 (By
REGION 267 390 Interaction with PLCG1 (By similarity).
REGION 405 572 G domain.
MOD_RES 149 149 Phosphoserine (By similarity).
MOD_RES 267 267 Phosphoserine (By similarity).
MOD_RES 638 638 Phosphoserine.
MOD_RES 808 808 Phosphoserine (By similarity).
MOD_RES 865 865 Phosphoserine (By similarity).
MOD_RES 927 927 Phosphoserine (By similarity). |
Keyword | 3D-structure; Alternative splicing; ANK repeat; Complete proteome; Cytoplasm; GTP-binding; GTPase activation; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Reference proteome; Repeat; Transport; Tumor suppressor; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1192 AA |
Protein Sequence | MSRGAGALQR RTTTYLISLT LVKLESVPPP PPSPSAAAVG APGARGSEPR DPGSPRGAEE 60
PGKKRHERLF HRQDALWIST SSAGAGGAEP PALSPAPASP ARPVSPAPGR RLSLWAAPPG 120
PPLSGGLSPD SKPGGAPSSS RRPLLSSPSW GGPEPEGRTG GGVPGSSSPH PGTGSRRLKV 180
APPPPAPKPC KTVTTSGAKA GGGKGAGSRL SWPESEGKPR VKGSKSSAGT GASVSAAATA 240
AAAGGGGSTA STSGGVGAGA GARGKLSPRK GKSKTLDNSD LHPGPPAGSP PPLTLPPTPS 300
PATAVTAASA QPPGPAPPIT LEPPAPGLKR GREGGRASTR DRKMLKFISG IFTKSTGGPP 360
GSGPLPGPPS LSSGSGSREL LGAELRASPK AVINSQEWTL SRSIPELRLG VLGDARSGKS 420
SLIHRFLTGS YQVLEKTESE QYKKEMLVDG QTHLVLIREE AGAPDAKFSG WADAVIFVFS 480
LEDENSFQAV SRLHGQLSSL RGEGRGGLAL ALVGTQDRIS ASSPRVVGDA RARALCADMK 540
RCSYYETCAT YGLNVDRVFQ EVAQKVVTLR KQQQLLAACK SLPSSPSHSA ASTPVAGQAS 600
NGGHTSDYSS SLPSSPNVGH RELRAEAAAV AGLSTPGSLH RAAKRRTSLF ANRRGSDSEK 660
RSLDSRGETT GSGRAIPIKQ SFLLKRSGNS LNKEWKKKYV TLSSNGFLLY HPSINDYIHS 720
THGKEMDLLR TTVKVPGKRP PRAISAFGPS ASINGLVKDM STVQMGEGLE ATTPMPSPSP 780
SPSSLQPPPD QTSKHLLKPD RNLARALSTD CTPSGDLSPL SREPPPSPMV KKQRRKKLTT 840
PSKTEGSAGQ AEAKRKMWKL KSFGSLRNIY KAEENFEFLI VSSTGQTWHF EAASFEERDA 900
WVQAIESQIL ASLQCCESSK VKLRTDSQSE AVAIQAIRNA KGNSICVDCG APNPTWASLN 960
LGALICIECS GIHRNLGTHL SRVRSLDLDD WPRELTLVLT AIGNDTANRV WESDTRGRAK 1020
PSRDSSREER ESWIRAKYEQ LLFLAPLSTS EEPLGRQLWA AVQAQDVATV LLLLAHARHG 1080
PLDTSVEDPQ LRSPLHLAAE LAHVVITQLL LWYGADVAAR DAQGRTALFY ARQAGSQLCA 1140
DILLQHGCPG EGGSAATTPS AATTPSITAT PSPRRRSSAA SVGRADAPVA LV 1192 |
Gene Ontology | GO:0005739; C:mitochondrion; IDA:HPA. GO:0005730; C:nucleolus; IDA:HPA. GO:0008060; F:ARF GTPase activator activity; IEA:InterPro. GO:0005525; F:GTP binding; IEA:UniProtKB-KW. GO:0005543; F:phospholipid binding; IEA:InterPro. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0007411; P:axon guidance; TAS:Reactome. GO:0043524; P:negative regulation of neuron apoptotic process; IGI:MGI. GO:0042177; P:negative regulation of protein catabolic process; IDA:MGI. GO:0043547; P:positive regulation of GTPase activity; IEA:GOC. GO:0015031; P:protein transport; IEA:UniProtKB-KW. GO:0032312; P:regulation of ARF GTPase activity; IEA:InterPro. GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |