CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015952
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Amyloid beta A4 precursor protein-binding family B member 1-interacting protein 
Protein Synonyms/Alias
 APBB1-interacting protein 1; Proline-rich EVH1 ligand 1; PREL-1; Proline-rich protein 73; Rap1-GTP-interacting adapter molecule; RIAM; Retinoic acid-responsive proline-rich protein 1; RARP-1 
Gene Name
 APBB1IP 
Gene Synonyms/Alias
 PREL1; RARP1; RIAM 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
164QAKADKIKLALEKLKubiquitination[1]
171KLALEKLKEAKVKKLubiquitination[1]
190HMNDNSTKSLMVDERubiquitination[1, 2]
254WTRDTENKILFLEKEubiquitination[1]
260NKILFLEKEEKYAVFubiquitination[1, 2]
268EEKYAVFKNPQNFYLubiquitination[1, 2, 3, 4]
344SGIYYVPKGKTKTSRubiquitination[1]
346IYYVPKGKTKTSRDLubiquitination[1]
375TQHKMKYKAPTDYCFubiquitination[1, 2]
385TDYCFVLKHPQIQKEubiquitination[1]
391LKHPQIQKESQYIKYubiquitination[1, 2]
397QKESQYIKYLCCDDTubiquitination[1, 2]
421IRIAKYGKTLYDNYQubiquitination[1, 3, 4]
433NYQRAVAKAGLASRWubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Appears to function in the signal transduction from Ras activation to actin cytoskeletal remodeling. Suppresses insulin- induced promoter activities through AP1 and SRE. Mediates Rap1- induced adhesion. 
Sequence Annotation
 DOMAIN 176 263 Ras-associating.
 DOMAIN 310 419 PH.
 MOD_RES 55 55 Phosphoserine.
 MOD_RES 526 526 Phosphoserine.
 MOD_RES 531 531 Phosphoserine (By similarity).  
Keyword
 3D-structure; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 666 AA 
Protein Sequence
MGESSEDIDQ MFSTLLGEMD LLTQSLGVDT LPPPDPNPPR AEFNYSVGFK DLNESLNALE 60
DQDLDALMAD LVADISEAEQ RTIQAQKESL QNQHHSASLQ ASIFSGAASL GYGTNVAATG 120
ISQYEDDLPP PPADPVLDLP LPPPPPEPLS QEEEEAQAKA DKIKLALEKL KEAKVKKLVV 180
KVHMNDNSTK SLMVDERQLA RDVLDNLFEK THCDCNVDWC LYEIYPELQI ERFFEDHENV 240
VEVLSDWTRD TENKILFLEK EEKYAVFKNP QNFYLDNRGK KESKETNEKM NAKNKESLLE 300
ESFCGTSIIV PELEGALYLK EDGKKSWKRR YFLLRASGIY YVPKGKTKTS RDLACFIQFE 360
NVNIYYGTQH KMKYKAPTDY CFVLKHPQIQ KESQYIKYLC CDDTRTLNQW VMGIRIAKYG 420
KTLYDNYQRA VAKAGLASRW TNLGTVNAAA PAQPSTGPKT GTTQPNGQIP QATHSVSAVL 480
QEAQRHAETS KDKKPALGNH HDPAVPRAPH APKSSLPPPP PVRRSSDTSG SPATPLKAKG 540
TGGGGLPAPP DDFLPPPPPP PPLDDPELPP PPPDFMEPPP DFVPPPPPSY AGIAGSELPP 600
PPPPPPAPAP APVPDSARPP PAVAKRPPVP PKRQENPGHP GGAGGGEQDF MSDLMKALQK 660
KRGNVS 666 
Gene Ontology
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000159; Ras-assoc. 
Pfam
 PF00169; PH
 PF00788; RA 
SMART
 SM00233; PH
 SM00314; RA 
PROSITE
 PS50003; PH_DOMAIN
 PS50200; RA 
PRINTS