CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-036920
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Gamma-interferon-inducible protein 16 
Protein Synonyms/Alias
  
Gene Name
 IFI16 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30DYHFRMVKSLLSNDLubiquitination[1]
38SLLSNDLKLNLKMREubiquitination[1, 2]
59IADLMEEKFRGDAGLubiquitination[1]
68RGDAGLGKLIKIFEDacetylation[2]
68RGDAGLGKLIKIFEDubiquitination[1, 2, 3]
87EDLAETLKKEKLKVKubiquitination[1, 3, 4]
88DLAETLKKEKLKVKGubiquitination[1]
94KKEKLKVKGPALSRKubiquitination[1, 3]
103PALSRKRKKEVDATSubiquitination[1, 2, 3, 5]
104ALSRKRKKEVDATSPubiquitination[1, 2, 3]
138QNPKTVAKCQVTPRRubiquitination[1, 2, 3]
150PRRNVLQKRPVIVKVubiquitination[1]
162VKVLSTTKPFEYETPubiquitination[1]
174ETPEMEKKIMFHATVubiquitination[1]
198KVLNTSLKEKFNGKKubiquitination[1, 2, 3, 4]
278HKKTVNQKTTIYEIQubiquitination[1, 2, 3, 5]
309IPCEEGDKLQLFCFRubiquitination[1]
325RKKNQMSKLISEMHSacetylation[2]
325RKKNQMSKLISEMHSubiquitination[1, 2]
348NPRNNDPKSMKLPQEacetylation[2]
391PSSSFFTKKSEDTISubiquitination[1, 3]
392SSSFFTKKSEDTISKubiquitination[1]
399KSEDTISKMNDFMRMubiquitination[1, 2, 3]
505SSFLTTLKPRLKTEPubiquitination[1]
509TTLKPRLKTEPEEVSubiquitination[1]
546YEPKEQKKMFHATVAubiquitination[3]
562ENEVFRVKVFNIDLKubiquitination[1, 2, 3]
569KVFNIDLKEKFTPKKacetylation[2]
569KVFNIDLKEKFTPKKubiquitination[1]
571FNIDLKEKFTPKKIIubiquitination[1]
576KEKFTPKKIIAIANYubiquitination[3]
611DRNMEIPKGLIRSASubiquitination[1]
622RSASVTPKINQLCSQubiquitination[1, 2, 3, 4]
631NQLCSQTKGSFVNGVubiquitination[1]
643NGVFEVHKKNVRGEFubiquitination[4]
680INCEEGDKLKLTCFEacetylation[2, 6]
680INCEEGDKLKLTCFEubiquitination[1, 3]
682CEEGDKLKLTCFELAacetylation[2]
682CEEGDKLKLTCFELAubiquitination[2, 3]
691TCFELAPKSGNTGELubiquitination[1, 2, 3, 4]
707SVIHSHIKVIKTRKNacetylation[2]
707SVIHSHIKVIKTRKNubiquitination[1]
715VIKTRKNKKDILNPDubiquitination[1, 3]
716IKTRKNKKDILNPDSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 733 AA 
Protein Sequence
MSVKMGKKYK NIVLLKGLEV INDYHFRMVK SLLSNDLKLN LKMREEYDKI QIADLMEEKF 60
RGDAGLGKLI KIFEDIPTLE DLAETLKKEK LKVKGPALSR KRKKEVDATS PAPSTSSTVK 120
TEGAEATPGA QNPKTVAKCQ VTPRRNVLQK RPVIVKVLST TKPFEYETPE MEKKIMFHAT 180
VATQTQFFHV KVLNTSLKEK FNGKKIIIIS DYLEYDSLLE VNEESTVSEA GPNQTFEVPN 240
KIINRAKETL KIDILHKQAS GNIVYGVFML HKKTVNQKTT IYEIQDDRGK MDVVGTGQCH 300
NIPCEEGDKL QLFCFRLRKK NQMSKLISEM HSFIQIKKKT NPRNNDPKSM KLPQEQRQLP 360
YPSEASTTFP ESHLRTPQMP PTTPSSSFFT KKSEDTISKM NDFMRMQILK EGSHFPGPFM 420
TSIGPAESHP HTPQMPPSTP SSSFLTTKSE DTISKMNDFM RMQILKEGSH FPGPFMTSIG 480
PAESHPHTPQ MPPSTPSSSF LTTLKPRLKT EPEEVSIEDS AQSDLKEVMV LNATESFVYE 540
PKEQKKMFHA TVATENEVFR VKVFNIDLKE KFTPKKIIAI ANYVCRNGFL EVYPFTLVAD 600
VNADRNMEIP KGLIRSASVT PKINQLCSQT KGSFVNGVFE VHKKNVRGEF TYYEIQDNTG 660
KMEVVVHGRL TTINCEEGDK LKLTCFELAP KSGNTGELRS VIHSHIKVIK TRKNKKDILN 720
PDSSMETSPD FFF 733 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA. 
Interpro
 IPR004020; DAPIN.
 IPR011029; DEATH-like_dom.
 IPR004021; HIN200/IF120x.
 IPR012340; NA-bd_OB-fold. 
Pfam
 PF02760; HIN
 PF02758; PYRIN 
SMART
  
PROSITE
 PS50824; DAPIN
 PS50834; HIN_200 
PRINTS