UniProt Accession

 TOP2B_MOUSE; Q64511; Q7TQG4 

Genbank Protein ID

 D38046; BC041106; BC054541 

Genbank Nucleotide ID

 BAA07236.1; AAH41106.1; AAH54541.1 

Protein Name

 DNA topoisomerase 2-beta 

Protein Synonyms/Alias

 DNA topoisomerase II, beta isozyme 

Gene Name

 Top2b 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
3	*****MAKSSLAGSD	acetylation	[1, 2]
21	TWVNNATKKEELETA	acetylation	[1]
538	YDDAESLKTLRYGKI	acetylation	[1]
1202	ILAGMSGKAIKGKVG	acetylation	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene (By similarity). 

Sequence Annotation

 DOMAIN 464 581 Toprim.
 NP_BIND 157 159 ATP (By similarity).
 NP_BIND 170 177 ATP (By similarity).
 NP_BIND 385 387 ATP (By similarity).
 REGION 351 353 Interaction with DNA (By similarity).
 REGION 999 1008 Interaction with DNA (By similarity).
 ACT_SITE 814 814 O-(5'-phospho-DNA)-tyrosine intermediate
 METAL 470 470 Magnesium 1; catalytic (By similarity).
 METAL 550 550 Magnesium 1; catalytic (By similarity).
 METAL 550 550 Magnesium 2 (By similarity).
 METAL 552 552 Magnesium 2 (By similarity).
 BINDING 100 100 ATP (By similarity).
 BINDING 129 129 ATP (By similarity).
 MOD_RES 419 419 Phosphoserine.
 MOD_RES 627 627 Phosphothreonine.
 MOD_RES 1224 1224 Phosphoserine (By similarity).
 MOD_RES 1280 1280 Phosphothreonine (By similarity).
 MOD_RES 1324 1324 Phosphoserine.
 MOD_RES 1328 1328 Phosphoserine.
 MOD_RES 1330 1330 Phosphoserine.
 MOD_RES 1332 1332 Phosphoserine.
 MOD_RES 1346 1346 Phosphoserine (By similarity).
 MOD_RES 1363 1363 Phosphoserine (By similarity).
 MOD_RES 1387 1387 Phosphoserine.
 MOD_RES 1390 1390 Phosphothreonine (By similarity).
 MOD_RES 1400 1400 Phosphoserine.
 MOD_RES 1408 1408 Phosphotyrosine (By similarity).
 MOD_RES 1411 1411 Phosphoserine (By similarity).
 MOD_RES 1428 1428 Phosphoserine (By similarity).
 MOD_RES 1439 1439 Phosphoserine (By similarity).
 MOD_RES 1441 1441 Phosphoserine (By similarity).
 MOD_RES 1448 1448 Phosphoserine (By similarity).
 MOD_RES 1453 1453 Phosphoserine.
 MOD_RES 1458 1458 Phosphothreonine.
 MOD_RES 1460 1460 Phosphoserine (By similarity).
 MOD_RES 1509 1509 Phosphoserine.
 MOD_RES 1511 1511 Phosphoserine.
 MOD_RES 1513 1513 Phosphoserine.
 MOD_RES 1537 1537 Phosphoserine.
 MOD_RES 1539 1539 Phosphoserine.
 MOD_RES 1562 1562 Phosphothreonine (By similarity).
 MOD_RES 1568 1568 Phosphoserine (By similarity).
 MOD_RES 1596 1596 Phosphotyrosine (By similarity).
 MOD_RES 1600 1600 Phosphoserine (By similarity).  

Keyword

 ATP-binding; Complete proteome; DNA-binding; Isomerase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Topoisomerase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1612 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005654; C:nucleoplasm; IEA:Compara.
 GO:0000795; C:synaptonemal complex; IBA:RefGenome.
 GO:0071778; C:WINAC complex; IBA:RefGenome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003682; F:chromatin binding; IBA:RefGenome.
 GO:0003918; F:DNA topoisomerase type II (ATP-hydrolyzing) activity; ISS:UniProtKB.
 GO:0042826; F:histone deacetylase binding; IBA:RefGenome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005080; F:protein kinase C binding; IBA:RefGenome.
 GO:0007409; P:axonogenesis; IMP:MGI.
 GO:0006265; P:DNA topological change; ISS:UniProtKB.
 GO:0006261; P:DNA-dependent DNA replication; IBA:RefGenome.
 GO:0030900; P:forebrain development; IMP:MGI.
 GO:0044774; P:mitotic DNA integrity checkpoint; IBA:RefGenome.
 GO:0006312; P:mitotic recombination; IBA:RefGenome.
 GO:0001764; P:neuron migration; IMP:MGI.
 GO:0000712; P:resolution of meiotic recombination intermediates; IBA:RefGenome.
 GO:0000819; P:sister chromatid segregation; IBA:RefGenome. 

Interpro

 IPR024946; Arg_repress_C-like.
 IPR012542; DTHCT.
 IPR003594; HATPase_ATP-bd.
 IPR020568; Ribosomal_S5_D2-typ_fold.
 IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
 IPR001241; Topo_IIA.
 IPR002205; Topo_IIA_A/C.
 IPR013758; Topo_IIA_A/C_ab.
 IPR013757; Topo_IIA_A_a.
 IPR013506; Topo_IIA_bsu_dom2.
 IPR013759; Topo_IIA_cen_dom.
 IPR013760; Topo_IIA_like_dom.
 IPR018522; TopoIIA_CS.
 IPR006171; Toprim_domain. 

Pfam

 PF00204; DNA_gyraseB
 PF00521; DNA_topoisoIV
 PF08070; DTHCT
 PF02518; HATPase_c
 PF01751; Toprim 

SMART

 SM00387; HATPase_c
 SM00433; TOP2c
 SM00434; TOP4c 

PROSITE

 PS00177; TOPOISOMERASE_II
 PS50880; TOPRIM 

PRINTS

 PR00418; TPI2FAMILY.