UniProt Accession

 PGAM5_MOUSE; Q8BX10; B2RSM6; Q3UK19; Q80VY8; Q8BM78; Q9CZU2 

Genbank Protein ID

 AK012159; AK034588; AK049246; AK146216; AK169643; BC052179; BC138924; BC138925 

Genbank Nucleotide ID

 BAB28067.1; BAC28763.1; BAC33634.1; BAE26984.1; BAE41272.1; AAH52179.1; AAI38925.1; AAI38926.1 

Protein Name

 Serine/threonine-protein phosphatase PGAM5, mitochondrial 

Protein Synonyms/Alias

 Phosphoglycerate mutase family member 5 

Gene Name

 Pgam5 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
92	TSRLDHYKAKATRHI	acetylation	[1]
94	RLDHYKAKATRHIFL	acetylation	[1]
140	RLASLGLKFNKIVHS	ubiquitination	[2]
190	DPPVSHWKPEAVQYY	acetylation	[1, 3]

Reference

 [1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2- dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore. 

Sequence Annotation

 REGION 76 81 Interaction with KEAP1 (By similarity).
 MOD_RES 79 79 Phosphoserine (By similarity).
 MOD_RES 115 115 N6-acetyllysine (By similarity).
 MOD_RES 143 143 N6-acetyllysine (By similarity).
 MOD_RES 190 190 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Hydrolase; Membrane; Mitochondrion; Mitochondrion outer membrane; Necrosis; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 288 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0004721; F:phosphoprotein phosphatase activity; IEA:EC. 

Interpro

 IPR013078; His_Pase_superF_clade-1. 

Pfam

 PF00300; His_Phos_1 

SMART

 SM00855; PGAM 

PROSITE

 PS00175; PG_MUTASE 

PRINTS