UniProt Accession

 HAUS1_HUMAN; Q96CS2; B2RDM7; Q8N837 

Genbank Protein ID

 AY360137; AK097403; AK315605; BC005958; BC014003 

Genbank Nucleotide ID

 AAQ63649.1; BAC05036.1; BAG37974.1; AAH05958.1; AAH14003.1 

Protein Name

 HAUS augmin-like complex subunit 1 

Protein Synonyms/Alias

 Coiled-coil domain-containing protein 5; Enhancer of invasion-cluster; HEI-C 

Gene Name

 HAUS1 

Gene Synonyms/Alias

 CCDC5; HEIC 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	QVAAWLKKIFGDHPI	ubiquitination	[1, 2, 3]
59	YLVIEDLKQKASEYE	ubiquitination	[2, 4, 5]
61	VIEDLKQKASEYESE	ubiquitination	[2]
132	DLFRTKSKSEEIKIE	ubiquitination	[2]
145	IELEKLEKNLTATLV	ubiquitination	[2]
155	TATLVLEKCLQEDVK	ubiquitination	[2]
162	KCLQEDVKKAELHLS	ubiquitination	[2]
163	CLQEDVKKAELHLST	ubiquitination	[2, 6]
186	RQNMDFLKAKSEEFR	ubiquitination	[2]
188	NMDFLKAKSEEFRFG	ubiquitination	[2]
197	EEFRFGIKAAEEQLS	ubiquitination	[2, 4, 6, 7]
223	SLVALSEKLARLKQQ	ubiquitination	[2, 4, 5, 6]
228	SEKLARLKQQTIPLK	ubiquitination	[2, 6]
236	QQTIPLKKKLESYLD	ubiquitination	[2]
237	QTIPLKKKLESYLDL	ubiquitination	[2, 5, 6]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. 

Sequence Annotation

  

Keyword

 Alternative splicing; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 278 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0070652; C:HAUS complex; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051297; P:centrosome organization; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0051225; P:spindle assembly; IMP:UniProtKB. 

Interpro

 IPR026243; HAUS1. 

Pfam

  

SMART

  

PROSITE

  

PRINTS

 PR02087; HAUSAUGMINL1.