CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001135
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Paired amphipathic helix protein Sin3b 
Protein Synonyms/Alias
 Histone deacetylase complex subunit Sin3b; Transcriptional corepressor Sin3b 
Gene Name
 SIN3B 
Gene Synonyms/Alias
 KIAA0700 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
239GQFLPEAKRSLFTGNubiquitination[1, 2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription (By similarity). 
Sequence Annotation
 DOMAIN 37 107 PAH 1.
 DOMAIN 153 238 PAH 2.
 DOMAIN 292 369 PAH 3.
 REGION 59 105 Interaction with REST (By similarity).
 REGION 284 540 Interaction with NCOR1 (By similarity).
 REGION 392 591 Interaction with SUDS3 and HDAC1 (By  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1162 AA 
Protein Sequence
MAHAGGGSGG SGAGGPAGRG LSGARWGRSG SAGHEKLPVH VEDALTYLDQ VKIRFGSDPA 60
TYNGFLEIMK EFKSQSIDTP GVIRRVSQLF HEHPDLIVGF NAFLPLGYRI DIPKNGKLNI 120
QSPLTSQENS HNHGDGAEDF KQQVPYKEDK PQVPLESDSV EFNNAISYVN KIKTRFLDHP 180
EIYRSFLEIL HTYQKEQLNT RGRPFRGMSE EEVFTEVANL FRGQEDLLSE FGQFLPEAKR 240
SLFTGNGPCE MHSVQKNEHD KTPEHSRKRS RPSLLRPVSA PAKKKMKLRG TKDLSIAAVG 300
KYGTLQEFSF FDKVRRVLKS QEVYENFLRC IALFNQELVS GSELLQLVSP FLGKFPELFA 360
QFKSFLGVKE LSFAPPMSDR SGDGISREID YASCKRIGSS YRALPKTYQQ PKCSGRTAIC 420
KELDHWTLLQ GSWTDDYCMS KFKNTCWIPG YSAGVLNDTW VSFPSWSEDS TFVSSKKTPY 480
EEQLHRCEDE RFELDVVLET NLATIRVLES VQKKLSRMAP EDQEKFRLDD SLGGTSEVIQ 540
RRAIYRIYGD KAPEIIESLK KNPVTAVPVV LKRLKAKEEE WREAQQGFNK IWREQYEKAY 600
LKSLDHQAVN FKQNDTKALR SKSLLNEIES VYDEHQEQHS EGRSAPSSEP HLIFVYEDRQ 660
ILEDAAALIS YYVKRQPAIQ KEDQGTIHQL LHQFVPSLFF SQQLDLGASE ESADEDRDSP 720
QGQTTDPSER KKPAPGPHSS PPEEKGAFGD APATEQPPLP PPAPHKPLDD VYSLFFANNN 780
WYFFLRLHQT LCSRLLKIYR QAQKQLLEYR TEKEREKLLC EGRREKGSDP AMELRLKQPS 840
EVELEEYYPA FLDMVRSLLE GSIDPTQYED TLREMFTIHA YVGFTMDKLV QNIARQLHHL 900
VSDDVCLKVV ELYLNEKKRG AAGGNLSSRC VRAARETSYQ WKAERCMADE NCFKVMFLQR 960
KGQVIMTIEL LDTEEAQTED PVEVQHLARY VEQYVGTEGA SSSPTEGFLL KPVFLQRNLK 1020
KFRRRWQSEQ ARALRGEARS SWKRLVGVES ACDVDCRFKL STHKMVFIVN SEDYMYRRGT 1080
LCRAKQVQPL VLLRHHQHFE EWHSRWLEDN VTVEAASLVQ DWLMGEEDED MVPCKTLCET 1140
VHVHGLPVTR YRVQYSRRPA SP 1162 
Gene Ontology
 GO:0030849; C:autosome; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000805; C:X chromosome; IEA:Compara.
 GO:0001741; C:XY body; IEA:Compara.
 GO:0000806; C:Y chromosome; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003714; F:transcription corepressor activity; IEA:Compara.
 GO:0048738; P:cardiac muscle tissue development; IEA:Compara.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:Compara.
 GO:0007519; P:skeletal muscle tissue development; IEA:Compara.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR013194; HDAC_interact.
 IPR003822; PAH. 
Pfam
 PF02671; PAH
 PF08295; Sin3_corepress 
SMART
 SM00761; HDAC_interact 
PROSITE
 PS51477; PAH 
PRINTS