UniProt Accession

 NEIL2_HUMAN; Q969S2; B4DFR7; Q7Z3Q7; Q8N842; Q8NG52 

Genbank Protein ID

 AB079070; AK056206; AK097389; AK294224; BX537529; AY306127; AC069185; BC013952; BC013964 

Genbank Nucleotide ID

 BAC06478.1; BAB71120.1; BAC05030.1; BAG57528.1; CAD97774.1; AAP45052.1; AAH13952.1; AAH13964.1 

Protein Name

 Endonuclease 8-like 2 

Protein Synonyms/Alias

 DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; Endonuclease VIII-like 2; Nei homolog 2; NEH2; Nei-like protein 2 

Gene Name

 NEIL2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
50	QDTQVHGKKLFLRFD	acetylation	[1]
96	VGEPSGQKTLDGSSR	ubiquitination	[2]
150	VNDFSRAKKANKRGD	acetylation	[1]
151	NDFSRAKKANKRGDW	acetylation	[1]
154	SRAKKANKRGDWRDP	acetylation	[1]
234	SGLGNIIKNEALYRA	ubiquitination	[2]
299	PAGHQVMKEAFGPED	ubiquitination	[3]

Reference

 [1] Acetylation of the human DNA glycosylase NEIL2 and inhibition of its activity.
 Bhakat KK, Hazra TK, Mitra S.
 Nucleic Acids Res. 2004;32(10):3033-9. [PMID: 15175427]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. 

Sequence Annotation

 ZN_FING 284 320 FPG-type.
 ACT_SITE 2 2 Schiff-base intermediate with DNA
 ACT_SITE 3 3 Proton donor (Probable).
 ACT_SITE 50 50 Proton donor; for beta-elimination
 ACT_SITE 310 310 Proton donor; for delta-elimination
 BINDING 231 231 DNA (By similarity).
 MOD_RES 50 50 N6-acetyllysine.
 MOD_RES 154 154 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Glycosidase; Hydrolase; Lyase; Metal-binding; Multifunctional enzyme; Nucleus; Polymorphism; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 332 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005876; C:spindle microtubule; IEA:Compara.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0003906; F:DNA-(apurinic or apyrimidinic site) lyase activity; IEA:EC.
 GO:0016799; F:hydrolase activity, hydrolyzing N-glycosyl compounds; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006284; P:base-excision repair; IEA:InterPro.
 GO:0006289; P:nucleotide-excision repair; IEA:InterPro. 

Interpro

 IPR015886; DNA_glyclase/AP_lyase_DNA-bd.
 IPR012319; DNA_glycosylase/AP_lyase_cat.
 IPR010979; Ribosomal_S13-like_H2TH.
 IPR000214; Znf_DNA_glyclase/AP_lyase. 

Pfam

 PF01149; Fapy_DNA_glyco
 PF06831; H2TH 

SMART

  

PROSITE

 PS51068; FPG_CAT
 PS01242; ZF_FPG_1
 PS51066; ZF_FPG_2 

PRINTS