CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039727
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Microtubule-actin cross-linking factor 1, isoforms 1/2/3/5 
Protein Synonyms/Alias
  
Gene Name
 MACF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
2239DRVQNLRKDFTELQKubiquitination[1]
4072LNEMKEFKVEVYQQQmethylation[2]
4122LWENLGEKIAHRQHKacetylation[3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Calcium; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 5300 AA 
Protein Sequence
MSSSDEETLS ERSCRSERSC RSERSYRSER SGSLSPCPPG DTLPWNLPLH EQKKRKSQDS 60
VLDPAERAVV RVADERDRVQ KKTFTKWVNK HLMKVRKHIN DLYEDLRDGH NLISLLEVLS 120
GIKLPREKGR MRFHRLQNVQ IALDFLKQRQ VKLVNIRNDD ITDGNPKLTL GLIWTIILHF 180
QISDIYISGE SGDMSAKEKL LLWTQKVTAG YTGIKCTNFS SCWSDGKMFN ALIHRYRPDL 240
VDMERVQIQS NRENLEQAFE VAERLGVTRL LDAEDVDVPS PDEKSVITYV SSIYDAFPKV 300
PEGGEGISAT EVDSRWQEYQ SRVDSLIPWI KQHTILMSDK TFPQNPVELK ALYNQYIHFK 360
ETEILAKERE KGRIEELYKL LEVWIEFGRI KLPQGYHPND VEEEWGKLII EMLEREKSLR 420
PAVERLELLL QIANKIQNGA LNCEEKLTLA KNTLQADAAH LESGQPVQCE SDVIMYIQEC 480
EGLIRQLQVD LQILRDENYY QLEELAFRVM RLQDELVTLR LECTNLYRKG HFTSLELVPP 540
STLTTTHLKA EPLTKATHSS STSWFRKPMT RAELVAISSS EDEGNLRFVY ELLSWVEEMQ 600
MKLERAEWGN DLPSVELQLE TQQHIHTSVE ELGSSVKEAR LYEGKMSQNF HTSYAETLGK 660
LETQYCKLKE TSSFRMRHLQ SLHKFVSRAT AELIWLNEKE EEELAYDWSD NNSNISAKRN 720
YFSELTMELE EKQDVFRSLQ DTAELLSLEN HPAKQTVEAY SAAVQSQLQW MKQLCLCVEQ 780
HVKENTAYFQ FFSDARELES FLRNLQDSIK RKYSCDHNTS LSRLEDLLQD SMDEKEQLIQ 840
SKSSVASLVG RSKTIVQLKP RSPDHVLKNT ISVKAVCDYR QIEITICKND ECVLEDNSQR 900
TKWKVISPTG NEAMVPSVCF LIPPPNKDAI EMASRVEQSY QKVMALWHQL HVNTKSLISW 960
NYLRKDLDLV QTWNLEKLRS SAPGECHQIM KNLQAHYEDF LQDSRDSVLF SVADRLRLEE 1020
EVEACKARFQ HLMKSMENED KEETVAKMYI SELKNIRLRL EEYEQRVVKR IQSLASSRTD 1080
RDAWQDNALR IAEQEHTQED LQQLRSDLDA VSMKCDSFLH QSPSSSSVPT LRSELNLLVE 1140
KMDHVYGLST VYLNKLKTVD VIVRSIQDAE LLVKGYEIKL SQEEVVLADL SALEAHWSTL 1200
RHWLSDVKDK NSVFSVLDEE IAKAKVVAEQ MSRLTPERNL DLERYQEKGS QLQERWHRVI 1260
AQLEIRQSEL ESIQEVLGDY RACHGTLIKW IEETTAQQEM MKPGQAEDSR VLSEQLSQQT 1320
ALFAEIERNQ TKLDQCQKFS QQYSTIVKDY ELQLMTYKAF VESQQKSPGK RRRMLSSSDA 1380
ITQEFMDLRT RYTALVTLTT QHVKYISDAL RRLEEEEKVV EEEKQEHVEK VKELLGWVST 1440
LARNTQGKAT SSETKESTDI EKAILEQQVL SEELTTKKEQ VSEAIKTSQI FLAKHGHKLS 1500
EKEKKQISEQ LNALNKAYHD LCDGSANQLQ QLQSQLAHQT EQKTLQKQQN TCHQQLEDLC 1560
SWVGQAERAL AGHQGRTTQQ DLSALQKNQS DLKDLQDDIQ NRATSFATVV KDIEGFMEEN 1620
QTKLSPRELT ALREKLHQAK EQYEALQEET RVAQKELEEA VTSALQQETE KSKAAKELAE 1680
NKKKIDALLD WVTSVGSSGG QLLTNLPGME QLSGASLEKG ALDTTDGYMG VNQAPEKLDK 1740
QCEMMKARHQ ELLSQQQNFI LATQSAQAFL DQHGHNLTPE EQQMLQQKLG ELKEQYSTSL 1800
AQSEAELKQV QTLQDELQKF LQDHKEFESW LERSEKELEN MHKGGSSPET LPSLLKRQGS 1860
FSEDVISHKG DLRFVTISGQ KVLDMENSFK EGKEPSEIGN LVKDKLKDAT ERYTALHSKC 1920
TRLGSHLNML LGQYHQFQNS ADSLQAWMQA CEANVEKLLS DTVASDPGVL QEQLATTKQL 1980
QEELAEHQVP VEKLQKVARD IMEIEGEPAP DHRHVQETTD SILSHFQSLS YSLAERSSLL 2040
QKAIAQSQSV QESLESLLQS IGEVEQNLEG KQVSSLSSGV IQEALATNMK LKQDIARQKS 2100
SLEATREMVT RFMETADSTT AAVLQGKLAE VSQRFEQLCL QQQEKESSLK KLLPQAEMFE 2160
HLSGKLQQFM ENKSRMLASG NQPDQDITHF FQQIQELNLE MEDQQENLDT LEHLVTELSS 2220
CGFALDLCQH QDRVQNLRKD FTELQKTVKE REKDASSCQE QLDEFRKLVR TFQKWLKETE 2280
GSIPPTETSM SAKELEKQIE HLKSLLDDWA SKGTLVEEIN CKGTSLENLI MEITAPDSQG 2340
KTDLTEIQCD MSDVNLKYEK LGGVLHERQE SLQAILNRME EVHKEANSVL QWLESKEEVL 2400
KSMDAMSSPT KTETVKAQAE SNKAFLAELE QNSPKIQKVK EALAGLLVTY PNSQEAENWK 2460
KIQEELNSRW ERATEVTVAR QRQLEESASH LACFQAAESQ LRPWLMEKEL MMGVLGPLSI 2520
DPNMLNAQKQ QVQFMLKEFE ARRQQHEQLN EAAQGILTGP GDVSLSTSQV QKELQSINQK 2580
WVELTDKLNS RSSQIDQAIV KSTQYQELLQ DLSEKVRAVG QRLSVQSAIS TQPEAVKQQL 2640
EETSEIRSDL EQLDHEVKEA QTLCDELSVL IGEQYLKDEL KKRLETVALP LQGLEDLAAD 2700
RINRLQAALA STQQFQQMFD ELRTWLDDKQ SQQAKNCPIS AKLERLQSQL QENEEFQKSL 2760
NQHSGSYEVI VAEGESLLLS VPPGEEKRTL QNQLVELKNH WEELSKKTAD RQSRLKDCMQ 2820
KAQKYQWHVE DLVPWIEDCK AKMSELRVTL DPVQLESSLL RSKAMLNEVE KRRSLLEILN 2880
SAADILINSS EADEDGIRDE KAGINQNMDA VTEELQAKTG SLEEMTQRLR EFQESFKNIE 2940
KKVEGAKHQL EIFDALGSQA CSNKNLEKLR AQQEVLQALE PQVDYLRNFT QGLVEDAPDG 3000
SDASQLLHQA EVAQQEFLEV KQRVNSGCVM MENKLEGIGQ FHCRVREMFS QLADLDDELD 3060
GMGAIGRDTD SLQSQIEDVR LFLNKIHVLK LDIEASEAEC RHMLEEEGTL DLLGLKRELE 3120
ALNKQCGKLT ERGKARQEQL ELTLGRVEDF YRKLKGLNDA TTAAEEAEAL QWVVGTEVEI 3180
INQQLADFKM FQKEQVDPLQ MKLQQVNGLG QGLIQSAGKD CDVQGLEHDM EEINARWNTL 3240
NKKVAQRIAQ LQEALLHCGK FQDALEPLLS WLADTEELIA NQKPPSAEYK VVKAQIQEQK 3300
LLQRLLDDRK ATVDMLQAEG GRIAQSAELA DREKITGQLE SLESRWTELL SKAAARQKQL 3360
EDILVLAKQF HETAEPISDF LSVTEKKLAN SEPVGTQTAK IQQQIIRHKA LNEEIVNRKK 3420
NVDQAIKNGQ ALLKQTTGEE VLLIQEKLDG IKTRYADITV TSSKALRTLE QARQLATKFQ 3480
STYEELTGWL REVEEELATS GGQSPTGEQI PQFQQRQKEL KKEVMEHRLV LDTVNEVSRA 3540
LLELVPWRAR EGLDKLVSDA NEQYKLVSDT IGQRVDEIDA AIQRSQQYEQ AADAELAWVA 3600
ETKRKLMALG PIRLEQDQTT AQLQVQKAFS IDIIRHKDSM DELFSHRSEI FGTCGEEQKT 3660
VLQEKTESLI QQYEAISLLN SERYARLERA QVLVNQFWET YEELSPWIEE TRALIAQLPS 3720
PAIDHEQLRQ QQEEMRQLRE SIAEHKPHID KLLKIGPQLK ELNPEEGEMV EEKYQKAENM 3780
YAQIKEEVRQ RALALDEAVS QSTQVSQFHD KIEPMLETLE NLSSRLRMPP LIPAEVDKIR 3840
ECISDNKSAT VELEKLQPSF EALKRRGEEL IGRSQGADKD LAAKEIQDKL DQMVFFWEDI 3900
KARAEEREIK FLDVLELAEK FWYDMAALLT TIKDTQDIVH DLESPGIDPS IIKQQVEAAE 3960
TIKEETDGLH EELEFIRILG ADLIFACGET EKPEVRKSID EMNNAWENLN KTWKERLEKL 4020
EDAMQAAVQY QDTLQAMFDW LDNTVIKLCT MPPVGTDLNT VKDQLNEMKE FKVEVYQQQI 4080
EMEKLNHQGE LMLKKATDET DRDIIREPLT ELKHLWENLG EKIAHRQHKL EGALLALGQF 4140
QHALEELMSW LTHTEELLDA QRPISGDPKV IEVELAKHHV LKNDVLAHQA TVETVNKAGN 4200
ELLESSAGDD ASSLRSRLEA MNQCWESVLQ KTEEREQQLQ STLQQAQGFH SEIEDFLLEL 4260
TRMESQLSAS KPTGGLPETA REQLDTHMEL YSQLKAKEET YNQLLDKGRL MLLSRDDSGS 4320
GSKTEQSVAL LEQKWHVVSS KMEERKSKLE EALNLATEFQ NSLQEFINWL TLAEQSLNIA 4380
SPPSLILNTV LSQIEEHKVF ANEVNAHRDQ IIELDQTGNQ LKFLSQKQDV VLIKNLLVSV 4440
QSRWEKVVQR SIERGRSLDD ARKRAKQFHE AWKKLIDWLE DAESHLDSEL EISNDPDKIK 4500
LQLSKHKEFQ KTLGGKQPVY DTTIRTGRAL KEKTLLPEDS QKLDNFLGEV RDKWDTVCGK 4560
SVERQHKLEE ALLFSGQFMD ALQALVDWLY KVEPQLAEDQ PVHGDLDLVM NLMDAHKVFQ 4620
KELGKRTGTV QVLKRSGREL IENSRDDTTW VKGQLQELST RWDTVCKLSV SKQSRLEQAL 4680
KQAEVFRDTV HMLLEWLSEA EQTLRFRGAL PDDTEALQSL IDTHKEFMKK VEEKRVDVNS 4740
AVAMGEVILA VCHPDCITTI KHWITIIRAR FEEVLTWAKQ HQQRLETALS ELVANAELLE 4800
ELLAWIQWAE TTLIQRDQEP IPQNIDRVKA LIAEHQTFME EMTRKQPDVD RVTKTYKRKN 4860
IEPTHAPFIE KSRSGGRKSL SQPTPPPMPI LSQSEAKNPR INQLSARWQQ VWLLALERQR 4920
KLNDALDRLE ELKEFANFDF DVWRKKYMRW MNHKKSRVMD FFRRIDKDQD GKITRQEFID 4980
GILASKFPTT KLEMTAVADI FDRDGDGYID YYEFVAALHP NKDAYRPTTD ADKIEDEVTR 5040
QVAQCKCAKR FQVEQIGENK YRFGDSQQLR LVRILRSTVM VRVGGGWMAL DEFLVKNDPC 5100
RARGRTNIEL REKFILPEGA SQGMTPFRSR GRRSKPSSRA ASPTRSSSSA SQSNHSCTSM 5160
PSSPATPASG TKVIPSSGSK LKRPTPTFHS SRTSLAGDTS NSSSPASTGA KTNRADPKKS 5220
ASRPGSRAGS RAGSRASSRR GSDASDFDLL ETQSACSDTS ESSAAGGQGN SRRGLNKPSK 5280
IPTMSKKTTT ASPRTPGPKR 5300 
Gene Ontology
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0007050; P:cell cycle arrest; IEA:InterPro. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR003108; GAS2_dom.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF13499; EF_hand_5
 PF02187; GAS2
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00054; EFh
 SM00243; GAS2
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51460; GAR 
PRINTS