| Tag | Content |
|---|
| CPLM ID | CPLM-012210 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Spliceosome RNA helicase DDX39B |
Protein Synonyms/Alias | 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein |
Gene Name | DDX39B |
Gene Synonyms/Alias | BAT1; UAP56 |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 36 | APAKKDVKGSYVSIH | ubiquitination | [1] | | 53 | GFRDFLLKPELLRAI | ubiquitination | [1] | | 349 | LSRYQQFKDFQRRIL | ubiquitination | [1] | | 399 | RAGRFGTKGLAITFV | ubiquitination | [1] |
|
Reference | [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA. Mol Cell Proteomics. 2012 May;11(5):148-59. [ PMID: 22505724] |
Functional Description | Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. |
Sequence Annotation | DOMAIN 76 249 Helicase ATP-binding.
DOMAIN 261 422 Helicase C-terminal.
NP_BIND 89 96 ATP.
MOTIF 45 73 Q motif.
MOTIF 196 199 DECD box.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 36 36 N6-acetyllysine.
MOD_RES 38 38 Phosphoserine.
MOD_RES 172 172 Phosphothreonine. |
Keyword | 3D-structure; Acetylation; Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; mRNA processing; mRNA splicing; mRNA transport; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Spliceosome; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 428 AA |
Protein Sequence | MAENDVDNEL LDYEDDEVET AAGGDGAEAP AKKDVKGSYV SIHSSGFRDF LLKPELLRAI 60
VDCGFEHPSE VQHECIPQAI LGMDVLCQAK SGMGKTAVFV LATLQQLEPV TGQVYLGRVL 120
GRGFWLGLVS VLVMCHTREL AFQISKEYER FSKYMPNVKV AVFFGGLSIK KDEEVLKKNC 180
PHIVVGTPGR ILALARNKSL NLKHIKHFIL DECDKMLEQL DMRRDVQEIF RMTPHEKQVM 240
MFSATLSKEI RPVCRKFMQD PMEIFVDDET KLTLHGLQQY YVKLKDNEKN RKLFDLLDVL 300
EFNQVVIFVK SVQRCIALAQ LLVEQNFPAI AIHRGMPQEE RLSRYQQFKD FQRRILVATN 360
LFGRGMDIER VNIAFNYDMP EDSDTYLHRV ARAGRFGTKG LAITFVSDEN DAKILNDVQD 420
RFEVNISELP DEIDISSYIE QTR 443 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell. GO:0005681; C:spliceosomal complex; IDA:BHF-UCL. GO:0000346; C:transcription export complex; IDA:UniProtKB. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0043008; F:ATP-dependent protein binding; IDA:BHF-UCL. GO:0004004; F:ATP-dependent RNA helicase activity; IDA:UniProtKB. GO:0030621; F:U4 snRNA binding; IDA:BHF-UCL. GO:0017070; F:U6 snRNA binding; IDA:BHF-UCL. GO:0046784; P:intronless viral mRNA export from host nucleus; IDA:UniProtKB. GO:0010501; P:RNA secondary structure unwinding; IDA:BHF-UCL. GO:0000245; P:spliceosomal complex assembly; IDA:BHF-UCL. |
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