UniProt Accession

 ACOC_MOUSE; P28271; Q3TQ15; Q99K54 

Genbank Protein ID

 X61147; AK163985; AL831793; CH466538; BC005454 

Genbank Nucleotide ID

 CAA43455.1; BAE37570.1; CAM27573.1; EDL05446.1; AAH05454.1 

Protein Name

 Cytoplasmic aconitate hydratase 

Protein Synonyms/Alias

 Aconitase; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1 

Gene Name

 Aco1 

Gene Synonyms/Alias

 Ireb1; Irebp 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
2	******MKNPFAHLA	ubiquitination	[1]
19	LDAAQPGKRFFNLNK	ubiquitination	[1]
26	KRFFNLNKLEDSRYG	acetylation	[2]
26	KRFFNLNKLEDSRYG	ubiquitination	[1]
56	NCDEFLVKKNDIENI	ubiquitination	[1]
79	KNIEVPFKPARVILQ	ubiquitination	[1]
141	RRADSLQKNQDLEFE	ubiquitination	[1]
379	GPKRPQDKVAVSEMK	ubiquitination	[1]
396	FESCLGAKQGFKGFQ	acetylation	[3]
396	FESCLGAKQGFKGFQ	ubiquitination	[1]
455	GAGLLAKKAVEAGLS	ubiquitination	[1]
464	VEAGLSVKPYIKTSL	ubiquitination	[1]
572	TVRIDFEKEPLGVNA	ubiquitination	[1]
610	HVIPGMFKEVYQKIE	ubiquitination	[1]
615	MFKEVYQKIETVNKS	acetylation	[2, 3, 4, 5]
615	MFKEVYQKIETVNKS	ubiquitination	[1]
621	QKIETVNKSWNALAA	ubiquitination	[1]
632	ALAAPSEKLYAWNPK	ubiquitination	[1]
639	KLYAWNPKSTYIKSP	acetylation	[6]
639	KLYAWNPKSTYIKSP	succinylation	[6]
639	KLYAWNPKSTYIKSP	ubiquitination	[1]
644	NPKSTYIKSPPFFES	ubiquitination	[1]
732	ANIRLLNKFLNKQAP	ubiquitination	[1]
736	LLNKFLNKQAPQTVH	ubiquitination	[1]
772	PLIVLAGKEYGSGSS	ubiquitination	[1]
785	SSRDWAAKGPFLLGI	ubiquitination	[1]
844	INIPEDLKPRMTVQI	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding (By similarity). 

Sequence Annotation

 REGION 205 207 Substrate binding (By similarity).
 REGION 779 780 Substrate binding (By similarity).
 METAL 437 437 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 503 503 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 506 506 Iron-sulfur (4Fe-4S) (By similarity).
 BINDING 86 86 Substrate (By similarity).
 BINDING 536 536 Substrate (By similarity).
 BINDING 541 541 Substrate (By similarity).
 BINDING 699 699 Substrate (By similarity).  

Keyword

 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome; RNA-binding; Tricarboxylic acid cycle. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 889 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005783; C:endoplasmic reticulum; IEA:Compara.
 GO:0005794; C:Golgi apparatus; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; ISS:UniProtKB.
 GO:0003994; F:aconitate hydratase activity; ISS:UniProtKB.
 GO:0052632; F:citrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0030350; F:iron-responsive element binding; ISS:UniProtKB.
 GO:0052633; F:isocitrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006879; P:cellular iron ion homeostasis; IMP:MGI.
 GO:0006101; P:citrate metabolic process; ISS:UniProtKB.
 GO:0050892; P:intestinal absorption; IGI:MGI.
 GO:0009791; P:post-embryonic development; IGI:MGI.
 GO:0006417; P:regulation of translation; IMP:MGI.
 GO:0010040; P:response to iron(II) ion; ISS:UniProtKB.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015928; Aconitase/3IPM_dehydase_swvl.
 IPR006249; Aconitase/Fe_reg_prot_2.
 IPR015934; Aconitase/Fe_reg_prot_2/AcnD.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS.
 IPR000573; AconitaseA/IPMdHydase_ssu_swvl. 

Pfam

 PF00330; Aconitase
 PF00694; Aconitase_C 

SMART

  

PROSITE

 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 

PRINTS

 PR00415; ACONITASE.