UniProt Accession

 UBF1_HUMAN; P17480; A8K6R8 

Genbank Protein ID

 X53390; X53461; X56687; AK291733; AK292518; BC042297 

Genbank Nucleotide ID

 CAA37469.1; CAA37548.1; CAA40016.1; BAF84422.1; BAF85207.1; AAH42297.1 

Protein Name

 Nucleolar transcription factor 1 

Protein Synonyms/Alias

 Autoantigen NOR-90; Upstream-binding factor 1; UBF-1 

Gene Name

 UBTF 

Gene Synonyms/Alias

 UBF; UBF1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	DLEMAAPKGQDRWSQ	ubiquitination	[1]
45	LPSNDSSKFKTTESH	ubiquitination	[1]
47	SNDSSKFKTTESHMD	ubiquitination	[1]
57	ESHMDWEKVAFKDFS	ubiquitination	[1]
61	DWEKVAFKDFSGDMC	ubiquitination	[1]
69	DFSGDMCKLKWVEIS	ubiquitination	[1]
71	SGDMCKLKWVEISNE	ubiquitination	[1, 2, 3]
97	LDAQEHVKNPYKGKK	ubiquitination	[1]
114	KHPDFPKKPLTPYFR	ubiquitination	[1]
126	YFRFFMEKRAKYAKL	ubiquitination	[1]
132	EKRAKYAKLHPEMSN	ubiquitination	[1, 2, 3]
144	MSNLDLTKILSKKYK	ubiquitination	[1]
151	KILSKKYKELPEKKK	ubiquitination	[1]
160	LPEKKKMKYIQDFQR	ubiquitination	[1, 2, 4]
191	PDLIQNAKKSDIPEK	ubiquitination	[1]
212	LWYTHEKKVYLKVRP	ubiquitination	[1]
216	HEKKVYLKVRPDATT	ubiquitination	[1, 2]
224	VRPDATTKEVKDSLG	ubiquitination	[1]
244	LSDKKRLKWIHKALE	ubiquitination	[1]
254	HKALEQRKEYEEIMR	ubiquitination	[1]
266	IMRDYIQKHPELNIS	ubiquitination	[1, 2, 5]
279	ISEEGITKSTLTKAE	ubiquitination	[1, 2, 5]
284	ITKSTLTKAERQLKD	ubiquitination	[1]
333	VLCSQQWKLLSQKEK	ubiquitination	[1, 3]
351	HKKCDQKKKDYEVEL	ubiquitination	[1]
352	KKCDQKKKDYEVELL	acetylation	[6]
352	KKCDQKKKDYEVELL	ubiquitination	[1]
378	QRVLGEEKMLNINKK	ubiquitination	[1]
384	EKMLNINKKQATSPA	ubiquitination	[1]
385	KMLNINKKQATSPAS	ubiquitination	[1]
452	WNDLSEKKKAKYKAR	ubiquitination	[1]
480	GEREERGKLPESPKR	ubiquitination	[1]
594	ELNHLPLKERMVEIG	ubiquitination	[1]
627	EEQQKQYKVHLDLWV	ubiquitination	[1]
646	PQDRAAYKEYISNKR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Dysregulation of upstream binding factor-1 acetylation at K352 is linked to impaired ribosomal DNA transcription in Huntington's disease.
 Lee J, Hwang YJ, Boo JH, Han D, Kwon OK, Todorova K, Kowall NW, Kim Y, Ryu H.
 Cell Death Differ. 2011 Nov;18(11):1726-35. [PMID: 21546905] 

Functional Description

 Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. 

Sequence Annotation

 DNA_BIND 112 180 HMG box 1.
 DNA_BIND 196 264 HMG box 2.
 DNA_BIND 298 362 HMG box 3.
 DNA_BIND 407 475 HMG box 4.
 DNA_BIND 482 549 HMG box 5.
 DNA_BIND 568 634 HMG box 6.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 201 201 Phosphothreonine.
 MOD_RES 273 273 Phosphoserine (By similarity).
 MOD_RES 336 336 Phosphoserine (By similarity).
 MOD_RES 364 364 Phosphoserine (By similarity).
 MOD_RES 389 389 Phosphoserine (By similarity).
 MOD_RES 412 412 Phosphoserine.
 MOD_RES 433 433 Phosphoserine (By similarity).
 MOD_RES 484 484 Phosphoserine.
 MOD_RES 495 495 Phosphoserine.
 MOD_RES 546 546 Phosphoserine (By similarity).
 MOD_RES 584 584 Phosphoserine (By similarity).
 MOD_RES 638 638 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 764 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0001832; P:blastocyst growth; IEA:Compara.
 GO:0007566; P:embryo implantation; IEA:Compara.
 GO:0045943; P:positive regulation of transcription from RNA polymerase I promoter; ISS:UniProtKB.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome. 

Interpro

 IPR009071; HMG_box_dom. 

Pfam

 PF09011; DUF1898
 PF00505; HMG_box 

SMART

 SM00398; HMG 

PROSITE

 PS50118; HMG_BOX_2 

PRINTS