UniProt Accession

 ADT1_MOUSE; P48962; Q62164 

Genbank Protein ID

 U27315; X74510; AF240002; BC003791; BC026925 

Genbank Nucleotide ID

 AAC52837.1; CAA52616.1; AAF64470.1; AAH03791.1; AAH26925.1 

Protein Name

 ADP/ATP translocase 1 

Protein Synonyms/Alias

 ADP,ATP carrier protein 1; ADP,ATP carrier protein, heart/skeletal muscle isoform T1; Adenine nucleotide translocator 1; ANT 1; Solute carrier family 25 member 4; mANC1 

Gene Name

 Slc25a4 

Gene Synonyms/Alias

 Anc1; Ant1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
10	DQALSFLKDFLAGGI	ubiquitination	[1, 2]
23	GIAAAVSKTAVAPIE	acetylation	[3, 4, 5, 6, 7, 8]
23	GIAAAVSKTAVAPIE	succinylation	[7]
23	GIAAAVSKTAVAPIE	ubiquitination	[2]
33	VAPIERVKLLLQVQH	acetylation	[3, 9]
33	VAPIERVKLLLQVQH	ubiquitination	[2]
43	LQVQHASKQISAEKQ	acetylation	[4, 7]
43	LQVQHASKQISAEKQ	succinylation	[7]
43	LQVQHASKQISAEKQ	ubiquitination	[2]
52	ISAEKQYKGIIDCVV	acetylation	[9]
63	DCVVRIPKEQGFLSF	acetylation	[3, 4, 6, 7]
63	DCVVRIPKEQGFLSF	succinylation	[7]
63	DCVVRIPKEQGFLSF	ubiquitination	[2]
92	QALNFAFKDKYKQIF	acetylation	[3, 4, 6, 7, 8, 9, 10, 11, 12, 13]
92	QALNFAFKDKYKQIF	phosphoglycerylation	[14]
92	QALNFAFKDKYKQIF	succinylation	[7]
92	QALNFAFKDKYKQIF	ubiquitination	[2]
94	LNFAFKDKYKQIFLG	acetylation	[9]
96	FAFKDKYKQIFLGGV	acetylation	[3, 4, 6, 7, 13]
96	FAFKDKYKQIFLGGV	phosphoglycerylation	[14]
96	FAFKDKYKQIFLGGV	ubiquitination	[2]
147	RLAADVGKGSSQREF	acetylation	[7, 8]
147	RLAADVGKGSSQREF	succinylation	[7]
147	RLAADVGKGSSQREF	ubiquitination	[2]
163	GLGDCLTKIFKSDGL	acetylation	[3, 4]
166	DCLTKIFKSDGLKGL	acetylation	[3, 4, 6, 7]
166	DCLTKIFKSDGLKGL	succinylation	[7]
166	DCLTKIFKSDGLKGL	ubiquitination	[2]
199	FGVYDTAKGMLPDPK	acetylation	[4, 6]
245	MMMQSGRKGADIMYT	acetylation	[7]
245	MMMQSGRKGADIMYT	succinylation	[7]
263	DCWRKIAKDEGANAF	acetylation	[3, 4, 7]
263	DCWRKIAKDEGANAF	succinylation	[7]
263	DCWRKIAKDEGANAF	ubiquitination	[2]
272	EGANAFFKGAWSNVL	acetylation	[3, 4, 7, 8, 9, 11, 12]
272	EGANAFFKGAWSNVL	succinylation	[7]
272	EGANAFFKGAWSNVL	succinylation	[7]
272	EGANAFFKGAWSNVL	ubiquitination	[2]

Reference

 [1] A proteomics approach to identify the ubiquitinated proteins in mouse heart.
 Jeon HB, Choi ES, Yoon JH, Hwang JH, Chang JW, Lee EK, Choi HW, Park ZY, Yoo YJ.
 Biochem Biophys Res Commun. 2007 Jun 8;357(3):731-6. [PMID: 17451654]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [8] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [9] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [10] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [11] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [12] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [13] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [14] Functional lysine modification by an intrinsically reactive primary glycolytic metabolite.
 Moellering RE, Cravatt BF.
 Science. 2013 Aug 2;341(6145):549-53. [PMID: 23908237] 

Functional Description

 Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane. 

Sequence Annotation

 REPEAT 6 98 Solcar 1.
 REPEAT 111 201 Solcar 2.
 REPEAT 212 297 Solcar 3.
 MOTIF 235 240 Substrate recognition (By similarity).
 BINDING 80 80 Nucleotide (By similarity).
 MOD_RES 2 2 N-acetylglycine (By similarity).
 MOD_RES 126 126 Phosphothreonine.
 MOD_RES 127 127 Phosphoserine.
 MOD_RES 191 191 Phosphotyrosine.
 MOD_RES 195 195 Phosphotyrosine.
 CROSSLNK 10 10 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Isopeptide bond; Membrane; Mitochondrion; Mitochondrion inner membrane; Phosphoprotein; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 298 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005741; C:mitochondrial outer membrane; ISS:MGI.
 GO:0005215; F:transporter activity; IEA:InterPro.
 GO:0008637; P:apoptotic mitochondrial changes; IGI:MGI.
 GO:0060547; P:negative regulation of necrotic cell death; IEA:Compara.
 GO:0055085; P:transmembrane transport; IEA:InterPro. 

Interpro

 IPR002113; Aden_trnslctor.
 IPR002067; Mit_carrier.
 IPR018108; Mitochondrial_sb/sol_carrier.
 IPR023395; Mt_carrier_dom. 

Pfam

 PF00153; Mito_carr 

SMART

  

PROSITE

 PS50920; SOLCAR 

PRINTS

 PR00927; ADPTRNSLCASE.
 PR00926; MITOCARRIER.