UniProt Accession

 GNL3L_HUMAN; Q9NVN8 

Genbank Protein ID

 AK001475; AL391139; BC011720 

Genbank Nucleotide ID

 BAA91712.1; CAI40396.1; AAH11720.1 

Protein Name

 Guanine nucleotide-binding protein-like 3-like protein 

Protein Synonyms/Alias

  

Gene Name

 GNL3L 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
61	NREAELKKKWVEEMR	ubiquitination	[1]
122	LDDEATRKAYYKEFR	ubiquitination	[1]
174	KLVLVLNKIDLVPKE	ubiquitination	[1]
185	VPKEVVEKWLDYLRN	ubiquitination	[1]
200	ELPTVAFKASTQHQV	ubiquitination	[1]
208	ASTQHQVKNLNRCSV	ubiquitination	[1]
226	QASESLLKSKACFGA	ubiquitination	[1]
228	SESLLKSKACFGAEN	ubiquitination	[1, 2, 3, 4]
265	VGLPNVGKSSLINSL	ubiquitination	[1, 5]
273	SSLINSLKRSRACSV	ubiquitination	[1, 2, 3]
288	GAVPGITKFMQEVYL	ubiquitination	[1, 2]
297	MQEVYLDKFIRLLDA	ubiquitination	[1, 2, 3, 5]
327	RNCVHVQKLADPVTP	ubiquitination	[1, 2, 3]
375	HRLGKKKKGGLYSQE	ubiquitination	[1]
386	YSQEQAAKAVLADWV	ubiquitination	[1, 2, 5]
469	LLHSPMTKIADAIEN	ubiquitination	[1]
477	IADAIENKTTVYKIG	ubiquitination	[1, 3, 4, 5]
482	ENKTTVYKIGDLTGY	ubiquitination	[1, 4, 6]
502	RHQMGWAKRNVDHRP	acetylation	[7]
502	RHQMGWAKRNVDHRP	ubiquitination	[1]
546	QALASALKNKKKMQK	ubiquitination	[1]
548	LASALKNKKKMQKRA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP. 

Sequence Annotation

 DOMAIN 264 320 G.
 NP_BIND 173 176 GTP (Potential).
 NP_BIND 259 266 GTP (Potential).
 NP_BIND 303 306 GTP (Potential).
 REGION 9 35 Required for nucleolar localization.  

Keyword

 Coiled coil; Complete proteome; GTP-binding; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; Ribosome biogenesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 582 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IBA:RefGenome.
 GO:0042254; P:ribosome biogenesis; IBA:RefGenome. 

Interpro

 IPR006073; GTP_binding_domain.
 IPR027417; P-loop_NTPase. 

Pfam

 PF01926; MMR_HSR1 

SMART

  

PROSITE

  

PRINTS

 PR00326; GTP1OBG.