UniProt Accession

 NSF1C_HUMAN; Q9UNZ2; A2A2L1; B2RD74; Q5JXA4; Q5JXA5; Q7Z533; Q9H102; Q9NVL9; Q9UI06 

Genbank Protein ID

 AF086909; AF112211; AF078856; AK297403; AK001511; AK315433; AL109658; AL109658; AL109658; AL109658; CH471133; CH471133; BC002801 

Genbank Nucleotide ID

 AAP97139.1; AAF17199.1; AAD44488.1; BAG59842.1; BAA91731.1; BAG37821.1; CAB96827.1; CAI22730.1; CAI22731.1; CAM28306.1; EAX10629.1; EAX10631.1; AAH02801.1 

Protein Name

 NSFL1 cofactor p47 

Protein Synonyms/Alias

 UBX domain-containing protein 2C; p97 cofactor p47 

Gene Name

 NSFL1C 

Gene Synonyms/Alias

 UBXN2C 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
112	QIVGPPRKKSPNELV	ubiquitination	[1, 2]
113	IVGPPRKKSPNELVD	ubiquitination	[2, 3]
124	ELVDDLFKGAKEHGA	ubiquitination	[1, 2, 3]
127	DDLFKGAKEHGAVAV	ubiquitination	[2]
146	KSPGETSKPRPFAGG	ubiquitination	[2, 3]
172	SAYVAGEKRQHSSQD	ubiquitination	[1, 2, 4, 5]
188	HVVLKLWKSGFSLDN	ubiquitination	[2, 3]
251	VKPKGAFKAFTGEGQ	ubiquitination	[2]
259	AFTGEGQKLGSTAPQ	ubiquitination	[2]
282	QQAENEAKASSSILI	ubiquitination	[2]
356	ADESQTLKEANLLNA	ubiquitination	[2]

Reference

 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). 

Sequence Annotation

 DOMAIN 179 244 SEP.
 DOMAIN 291 368 UBX.
 MOTIF 109 115 Nuclear localization signal.
 MOTIF 172 175 Nuclear localization signal.
 MOD_RES 74 74 Phosphoserine (By similarity).
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 140 140 Phosphoserine.
 MOD_RES 167 167 Phosphotyrosine (By similarity).
 MOD_RES 176 176 Phosphoserine (By similarity).
 MOD_RES 272 272 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Chromosome; Complete proteome; Direct protein sequencing; Golgi apparatus; Lipid-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 370 AA 

Protein Sequence

Gene Ontology

 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005795; C:Golgi stack; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW. 

Interpro

 IPR012989; SEP_domain.
 IPR009060; UBA-like.
 IPR001012; UBX. 

Pfam

 PF08059; SEP
 PF00789; UBX 

SMART

 SM00553; SEP
 SM00166; UBX 

PROSITE

 PS51399; SEP
 PS50033; UBX 

PRINTS