CPLM-012581

Genbank Nucleotide ID

Transcription initiation factor TFIID subunit 7

Protein Synonyms/Alias

RNA polymerase II TBP-associated factor subunit F; Transcription initiation factor TFIID 55 kDa subunit; TAF(II)55; TAFII-55; TAFII55

Homo sapiens (Human)

Position	Peptide	Type	References
5	***MSKSKDDAPHEL	methylation	[1]
5	***MSKSKDDAPHEL	ubiquitination	[2]
66	DRVPLASKLVDLPCV	ubiquitination	[3]
141	HGITLPLKNVRKRRF	ubiquitination	[4, 5, 6]
291	QIDNMKGKLQETQDR	acetylation	[3]

[1] Structural basis for the methylation site specificity of SET7/9.
Couture JF, Collazo E, Hauk G, Trievel RC.
Nat Struct Mol Biol. 2006 Feb;13(2):140-6. [PMID: 16415881]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively).

MOTIF 3 5 [KR]-[STA]-K motif.
MOD_RES 171 171 Phosphoserine.
MOD_RES 213 213 Phosphoserine.
MOD_RES 264 264 Phosphoserine.

Coiled coil; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005794; C:Golgi apparatus; IDA:HPA.
GO:0071339; C:MLL1 complex; IDA:UniProtKB.
GO:0005669; C:transcription factor TFIID complex; IDA:UniProtKB.
GO:0033276; C:transcription factor TFTC complex; IDA:UniProtKB.
GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
GO:0044212; F:transcription regulatory region DNA binding; IDA:UniProtKB.
GO:0030520; P:intracellular estrogen receptor signaling pathway; IEA:Compara.
GO:0035067; P:negative regulation of histone acetylation; IDA:UniProtKB.
GO:0006469; P:negative regulation of protein kinase activity; IDA:UniProtKB.
GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
GO:0000296; P:spermine transport; ISS:UniProtKB.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:UniProtKB.

IPR006751; TAFII55_prot_cons_reg.