CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039873
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Pre-mRNA-splicing factor SYF1 
Protein Synonyms/Alias
  
Gene Name
 XAB2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
33MRNQFSVKCWLRYIEubiquitination[1]
42WLRYIEFKQGAPKPRubiquitination[1]
59QLYERALKLLPCSYKubiquitination[1]
66KLLPCSYKLWYRYLKubiquitination[1]
171RGYRRFLKLSPESAEubiquitination[1, 2, 3]
185EEYIEYLKSSDRLDEubiquitination[1, 2, 3]
386VQTVDPFKATGKPHTubiquitination[1]
390DPFKATGKPHTLWVAubiquitination[1]
417DARVILEKATKVNFKacetylation[4, 5]
417DARVILEKATKVNFKubiquitination[1, 2, 5]
420VILEKATKVNFKQVDubiquitination[1]
455EALRLLRKATALPARubiquitination[1]
536KYFEESFKAYERGISubiquitination[2]
590GCPPKYAKTLYLLYAubiquitination[1]
634DMFNIYIKRAAEIYGubiquitination[1, 2, 3, 6]
651HTRGIYQKAIEVLSDubiquitination[1, 2, 3, 6, 7]
674RFADMECKLGEIDRAubiquitination[1, 2, 5, 6]
705GAFWQTWKDFEVRHGubiquitination[1, 2, 3, 7, 8]
718HGNEDTIKEMLRIRRubiquitination[1, 2, 3]
767QSGMDDMKLLEQRAEubiquitination[1, 2, 3, 6]
791QPLRAQSKILFVRSDubiquitination[1]
850AAVFGSLKED*****ubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 852 AA 
Protein Sequence
MARLSRPERP DLVFEEEDLP YEEEIMRNQF SVKCWLRYIE FKQGAPKPRL NQLYERALKL 60
LPCSYKLWYR YLKARRAQVK HRCVTDPAYE DVNNCHERAF VFMHKMPRLW LDYCQFLMDQ 120
GRVTHTRRTF DRALRALPIT QHSRIWPLYL RFLRSHPLPE TAVRGYRRFL KLSPESAEEY 180
IEYLKSSDRL DEAAQRLATV VNDERFVSKA GKSNYQLWHE LCDLISQNPD KVQSLNVDAI 240
IRGGLTRFTD QLGKLWCSLA DYYIRSGHFE KARDVYEEAI RTVMTVRDFT QVFDSYAQFE 300
ESMIAAKMET ASELGREEED DVDLELRLAR FEQLISRRPL LLNSVLLRQN PHHVHEWHKR 360
VALHQGRPRE IINTYTEAVQ TVDPFKATGK PHTLWVAFAK FYEDNGQLDD ARVILEKATK 420
VNFKQVDDLA SVWCQCGELE LRHENYDEAL RLLRKATALP ARRAEYFDGS EPVQNRVYKS 480
LKVWSMLADL EESLGTFQST KAVYDRILDL RIATPQIVIN YAMFLEEHKY FEESFKAYER 540
GISLFKWPNV SDIWSTYLTK FIARYGGRKL ERARDLFEQA LDGCPPKYAK TLYLLYAQLE 600
EEWGLARHAM AVYERATRAV EPAQQYDMFN IYIKRAAEIY GVTHTRGIYQ KAIEVLSDEH 660
AREMCLRFAD MECKLGEIDR ARAIYSFCSQ ICDPRTTGAF WQTWKDFEVR HGNEDTIKEM 720
LRIRRSVQAT YNTQVNFMAS QMLKVSGSAT GTVSDLAPGQ SGMDDMKLLE QRAEQLAAEA 780
ERDQPLRAQS KILFVRSDAS REELAELAQQ VNPEEIQLGE DEDEDEMDLE PNEVRLEQQS 840
VPAAVFGSLK ED 852 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0001824; P:blastocyst development; IEA:Compara.
 GO:0006396; P:RNA processing; IEA:InterPro. 
Interpro
 IPR003107; HAT.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 
Pfam
 PF07719; TPR_2 
SMART
 SM00386; HAT 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS