CPLM-004710

Genbank Nucleotide ID

Cyclic AMP-dependent transcription factor ATF-6 alpha

Protein Synonyms/Alias

cAMP-dependent transcription factor ATF-6 alpha; Activating transcription factor 6 alpha; ATF6-alpha; Processed cyclic AMP-dependent transcription factor ATF-6 alpha

Homo sapiens (Human)

Position	Peptide	Type	References
87	ICTVKDIKAEPQPLS	ubiquitination	[1, 2, 3, 4, 5]
176	KKIQVNSKPSIQPKP	ubiquitination	[3]
201	TNSSVPAKTIIIQTV	ubiquitination	[1, 3, 4]
216	PTLMPLAKQQPIISL	ubiquitination	[2, 3]
290	NGKLSVTKPVLQSTM	ubiquitination	[3]
339	LGLEARLKAALSENE	ubiquitination	[3]
349	LSENEQLKKENGTLK	ubiquitination	[3]
424	HLLGFSAKEAQDTSD	ubiquitination	[1, 2, 3, 4]
436	TSDGIIQKNSYRYDH	ubiquitination	[1, 2, 3, 4]
506	RMTNNQQKTRILQGA	ubiquitination	[4]
590	HNKTTRPKMSIVLPA	ubiquitination	[3]
629	DTRILHIKSSSVPPY	ubiquitination	[3, 5]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.

DOMAIN 306 369 bZIP.
REGION 1 150 Transcription activation.
REGION 308 339 Basic motif (By similarity).
REGION 348 355 Leucine-zipper (By similarity).
CARBOHYD 472 472 N-linked (GlcNAc...).
CARBOHYD 584 584 N-linked (GlcNAc...).
CARBOHYD 643 643 N-linked (GlcNAc...).

Activator; Complete proteome; DNA-binding; Endoplasmic reticulum; Glycoprotein; Membrane; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Signal-anchor; Transcription; Transcription regulation; Transmembrane; Transmembrane helix; Unfolded protein response.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
GO:0000139; C:Golgi membrane; TAS:Reactome.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005635; C:nuclear envelope; TAS:ProtInc.
GO:0005654; C:nucleoplasm; TAS:Reactome.
GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
GO:0006990; P:positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response; TAS:ProtInc.
GO:0006457; P:protein folding; TAS:ProtInc.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

PS50217; BZIP
PS00036; BZIP_BASIC