UniProt Accession

 RLME_ECOLI; P0C0R7; P28692; Q2M933 

Genbank Protein ID

 M83138; U01376; U18997; U00096; AP009048 

Genbank Nucleotide ID

 AAA23812.1; AAA97507.1; AAA57980.1; AAC76211.1; BAE77223.1 

Protein Name

 Ribosomal RNA large subunit methyltransferase E 

Protein Synonyms/Alias

 23S rRNA Um2552 methyltransferase; Cell division protein FtsJ; rRNA (uridine-2'-O-)-methyltransferase 

Gene Name

 rlmE 

Gene Synonyms/Alias

 ftsJ; mrsF; rrmJ; b3179; JW3146 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
21	LQEHFSDKYVQQAQK	acetylation	[1]
28	KYVQQAQKKGLRSRA	acetylation	[1]
38	LRSRAWFKLDEIQQS	acetylation	[1]
47	DEIQQSDKLFKPGMT	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit. 

Sequence Annotation

 ACT_SITE 164 164 Proton acceptor.
 BINDING 63 63 S-adenosyl-L-methionine; via amide
 BINDING 65 65 S-adenosyl-L-methionine; via amide
 BINDING 83 83 S-adenosyl-L-methionine.
 BINDING 99 99 S-adenosyl-L-methionine.
 BINDING 124 124 S-adenosyl-L-methionine.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Methyltransferase; Reference proteome; rRNA processing; S-adenosyl-L-methionine; Stress response; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 209 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008650; F:rRNA (uridine-2'-O-)-methyltransferase activity; IDA:EcoCyc.
 GO:0000453; P:enzyme-directed rRNA 2'-O-methylation; IMP:EcoCyc.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 

Interpro

 IPR015507; rRNA-MeTfrase_E.
 IPR002877; rRNA_MeTrfase_FtsJ_dom.
 IPR004512; rRNA_MeTrfase_gammaproteobac. 

Pfam

 PF01728; FtsJ 

SMART

  

PROSITE

  

PRINTS