UniProt Accession

 MED30_HUMAN; Q96HR3 

Genbank Protein ID

 AY083305; BC008226 

Genbank Nucleotide ID

 AAL89787.1; AAH08226.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 30 

Protein Synonyms/Alias

 Mediator complex subunit 30; TRAP/Mediator complex component TRAP25; Thyroid hormone receptor-associated protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; Trap25 

Gene Name

 MED30 

Gene Synonyms/Alias

 THRAP6; TRAP25 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
77	TYQDRLTKLQDNLRQ	ubiquitination	[1]
147	REIAEVNKKLKQKNQ	ubiquitination	[1]
148	EIAEVNKKLKQKNQQ	ubiquitination	[1]
157	KQKNQQLKQIMDQLR	ubiquitination	[2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.  

Keyword

 Acetylation; Activator; Coiled coil; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 178 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IDA:UniProtKB.
 GO:0030374; F:ligand-dependent nuclear receptor transcription coactivator activity; NAS:UniProtKB.
 GO:0004872; F:receptor activity; IDA:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IDA:UniProtKB.
 GO:0046966; F:thyroid hormone receptor binding; IDA:UniProtKB.
 GO:0042809; F:vitamin D receptor binding; NAS:UniProtKB.
 GO:0030521; P:androgen receptor signaling pathway; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IDA:UniProtKB. 

Interpro

 IPR021019; Mediator_Med30_met. 

Pfam

 PF11315; Med30 

SMART

  

PROSITE

  

PRINTS