CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006337
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein MSN4 
Protein Synonyms/Alias
 Multicopy suppressor of SNF1 protein 4 
Gene Name
 MSN4 
Gene Synonyms/Alias
 YKL062W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
108LATNWKWKNSNNAQFubiquitination[1]
121QFVNDGEKQSSNANGubiquitination[1]
298HKNSSNSKPTQQIIPubiquitination[1]
357SKDSGITKPKSISSSacetylation[2]
359DSGITKPKSISSSLNubiquitination[1]
557PVQPRKRKSITTIDPacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 
Functional Description
 Positive transcriptional factor that acts as a component of the stress responsive system. Recognizes and binds to the stress response element (STRE) which is involved in the response to various forms of stress (heat, oxidative, osmotic, etc.). Involved in the regulation of the CTT1, DDR2, HSP12 genes. 
Sequence Annotation
 ZN_FING 573 596 C2H2-type 1.
 ZN_FING 602 624 C2H2-type 2.
 MOD_RES 178 178 Phosphoserine.
 MOD_RES 263 263 Phosphoserine.
 MOD_RES 316 316 Phosphoserine.
 MOD_RES 319 319 Phosphoserine.
 MOD_RES 479 479 Phosphothreonine.
 MOD_RES 558 558 Phosphoserine.  
Keyword
 Activator; Complete proteome; Cytoplasm; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 630 AA 
Protein Sequence
MLVFGPNSSF VRHANKKQED SSIMNEPNGL MDPVLSTTNV SATSSNDNSA NNSISSPEYT 60
FGQFSMDSPH RTDATNTPIL TATTNTTANN SLMNLKDTAS LATNWKWKNS NNAQFVNDGE 120
KQSSNANGKK NGGDKIYSSV ATPQALNDEL KNLEQLEKVF SPMNPINDSH FNENIELSPH 180
QHATSPKTNL LEAEPSIYSN LFLDARLPNN ANSTTGLNDN DYNLDDTNND NTNSMQSILE 240
DFVSSEEALK FMPDAGRDAR RYSEVVTSSF PSMTDSRNSI SHSIEFWNLN HKNSSNSKPT 300
QQIIPEGTAT TERRGSTISP TTTINNSNPN FKLLDHDVSQ ALSGYSMDFS KDSGITKPKS 360
ISSSLNRISH SSSTTRQQRA SLPLIHDIES FANDSVMANP LSDSASFLSE ENEDDAFGAL 420
NYNSLDATTM SAFDNNVDPF NILKSSPAQD QQFIKPSMML SDNASAAAKL ATSGVDNITP 480
TPAFQRRSYD ISMNSSFKIL PTSQAHHAAQ HHQQQPTKQA TVSPNTRRRK SSSVTLSPTI 540
SHNNNNGKVP VQPRKRKSIT TIDPNNYDKN KPFKCKDCEK AFRRSEHLKR HIRSVHSTER 600
PFACMFCEKK FSRSDNLSQH LKTHKKHGDF 630 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IDA:SGD.
 GO:0000982; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity; IMP:SGD.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0001324; P:age-dependent response to oxidative stress involved in chronological cell aging; IGI:SGD.
 GO:0006338; P:chromatin remodeling; IGI:SGD.
 GO:0061401; P:positive regulation of transcription from RNA polymerase II promoter in response to a hypotonic environment; IGI:SGD.
 GO:0061402; P:positive regulation of transcription from RNA polymerase II promoter in response to acidity; IMP:SGD.
 GO:0061422; P:positive regulation of transcription from RNA polymerase II promoter in response to alkalinity; IGI:SGD.
 GO:0061412; P:positive regulation of transcription from RNA polymerase II promoter in response to amino acid starvation; IGI:SGD.
 GO:0061395; P:positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance; IGI:SGD.
 GO:0061410; P:positive regulation of transcription from RNA polymerase II promoter in response to ethanol; IGI:SGD.
 GO:0061409; P:positive regulation of transcription from RNA polymerase II promoter in response to freezing; IGI:SGD.
 GO:0061406; P:positive regulation of transcription from RNA polymerase II promoter in response to glucose starvation; IGI:SGD.
 GO:0061408; P:positive regulation of transcription from RNA polymerase II promoter in response to heat stress; IMP:SGD.
 GO:0061407; P:positive regulation of transcription from RNA polymerase II promoter in response to hydrogen peroxide; IMP:SGD.
 GO:0061405; P:positive regulation of transcription from RNA polymerase II promoter in response to hydrostatic pressure; IGI:SGD.
 GO:0061404; P:positive regulation of transcription from RNA polymerase II promoter in response to increased salt; IGI:SGD.
 GO:0061403; P:positive regulation of transcription from RNA polymerase II promoter in response to nitrosative stress; IGI:SGD.
 GO:0097236; P:positive regulation of transcription from RNA polymerase II promoter in response to zinc ion starvation; IGI:SGD.
 GO:0061434; P:regulation of replicative cell aging by regulation of transcription from RNA polymerase II promoter in response to caloric restriction; IGI:SGD.
 GO:0001302; P:replicative cell aging; IGI:SGD. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00096; zf-C2H2 
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS