UniProt Accession

 TPMT_HUMAN; P51580; O14806; O15423; O15424; O15425; O15426; O15515; O15548; O43213; Q5VUK6; Q9UBE6; Q9UBT8; Q9UE62 

Genbank Protein ID

 S62904; U12387; U30518; U30512; U30513; U30514; U30515; U30516; U30517; AF019369; AF019364; AF019365; AF019366; AF019367; AF019368; U81562; U81563; U81564; U81565; U81566; U81567; U81568; U81569; U81570; U81571; U81572; U81573; AB045146; AL589723; BC009596; AF035426; AF021876; AF021877 

Genbank Nucleotide ID

 AAB27277.1; AAC50130.1; AAC50368.1; AAC50368.1; AAC50368.1; AAC50368.1; AAC50368.1; AAC50368.1; AAC50368.1; AAC51865.1; AAC51865.1; AAC51865.1; AAC51865.1; AAC51865.1; AAC51865.1; AAB71625.1; AAB71626.1; AAB71627.1; AAB71628.1; AAB71629.1; AAB71630.1; AAB71631.1; AAB71632.1; AAB71633.1; AAB71634.1; AAB71635.1; AAB71636.1; BAA97037.1; CAH71233.1; AAH09596.1; AAC32289.1; AAB80746.1; AAB80747.1 

Protein Name

 Thiopurine S-methyltransferase 

Protein Synonyms/Alias

 Thiopurine methyltransferase 

Gene Name

 TPMT 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
37	QDKWVNGKTAFHQEQ	ubiquitination	[1, 2]
58	KHLDTFLKGKSGLRV	acetylation	[3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. 

Sequence Annotation

 REGION 29 40 S-adenosyl-L-methionine binding.
 REGION 134 135 S-adenosyl-L-methionine binding.
 BINDING 40 40 Substrate (By similarity).
 BINDING 69 69 S-adenosyl-L-methionine; via carbonyl
 BINDING 90 90 S-adenosyl-L-methionine.
 BINDING 152 152 S-adenosyl-L-methionine.
 MOD_RES 14 14 Phosphoserine.
 MOD_RES 58 58 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Methyltransferase; Phosphoprotein; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 245 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0008119; F:thiopurine S-methyltransferase activity; TAS:ProtInc.
 GO:0006139; P:nucleobase-containing compound metabolic process; TAS:ProtInc.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0006805; P:xenobiotic metabolic process; TAS:Reactome. 

Interpro

 IPR025835; Thiopurine_S-MeTrfase.
 IPR008854; TPMT. 

Pfam

 PF05724; TPMT 

SMART

  

PROSITE

  

PRINTS