CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006937
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Vitamin K-dependent gamma-carboxylase 
Protein Synonyms/Alias
 Gamma-glutamyl carboxylase; Peptidyl-glutamate 4-carboxylase; Vitamin K gamma glutamyl carboxylase 
Gene Name
 GGCX 
Gene Synonyms/Alias
 GC 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
91GLSSLDRKYLDGLDVubiquitination[1, 2]
318CSPEWPRKLVSYCPRubiquitination[3]
334LQQLLPLKAAPQPSVubiquitination[1, 2, 4, 5]
346PSVSCVYKRSRGKSGubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. 
Sequence Annotation
 ACT_SITE 218 218 Proton acceptor.
 MOD_RES 2 2 N-acetylalanine.
 CARBOHYD 459 459 N-linked (GlcNAc...).
 CARBOHYD 550 550 N-linked (GlcNAc...).
 DISULFID 99 450  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Disease mutation; Disulfide bond; Endoplasmic reticulum; Glycoprotein; Lyase; Membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 758 AA 
Protein Sequence
MAVSAGSART SPSSDKVQKD KAELISGPRQ DSRIGKLLGF EWTDLSSWRR LVTLLNRPTD 60
PASLAVFRFL FGFLMVLDIP QERGLSSLDR KYLDGLDVCR FPLLDALRPL PLDWMYLVYT 120
IMFLGALGMM LGLCYRISCV LFLLPYWYVF LLDKTSWNNH SYLYGLLAFQ LTFMDANHYW 180
SVDGLLNAHR RNAHVPLWNY AVLRGQIFIV YFIAGVKKLD ADWVEGYSME YLSRHWLFSP 240
FKLLLSEELT SLLVVHWGGL LLDLSAGFLL FFDVSRSIGL FFVSYFHCMN SQLFSIGMFS 300
YVMLASSPLF CSPEWPRKLV SYCPRRLQQL LPLKAAPQPS VSCVYKRSRG KSGQKPGLRH 360
QLGAAFTLLY LLEQLFLPYS HFLTQGYNNW TNGLYGYSWD MMVHSRSHQH VKITYRDGRT 420
GELGYLNPGV FTQSRRWKDH ADMLKQYATC LSRLLPKYNV TEPQIYFDIW VSINDRFQQR 480
IFDPRVDIVQ AAWSPFQRTS WVQPLLMDLS PWRAKLQEIK SSLDNHTEVV FIADFPGLHL 540
ENFVSEDLGN TSIQLLQGEV TVELVAEQKN QTLREGEKMQ LPAGEYHKVY TTSPSPSCYM 600
YVYVNTTELA LEQDLAYLQE LKEKVENGSE TGPLPPELQP LLEGEVKGGP EPTPLVQTFL 660
RRQQRLQEIE RRRNTPFHER FFRFLLRKLY VFRRSFLMTC ISLRNLILGR PSLEQLAQEV 720
TYANLRPFEA VGELNPSNTD SSHSNPPESN PDPVHSEF 758 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0008488; F:gamma-glutamyl carboxylase activity; TAS:ProtInc.
 GO:0007596; P:blood coagulation; TAS:ProtInc.
 GO:0017187; P:peptidyl-glutamic acid carboxylation; TAS:Reactome.
 GO:0043687; P:post-translational protein modification; TAS:Reactome. 
Interpro
 IPR011020; HTTM.
 IPR014710; RmlC-like_jellyroll.
 IPR011051; RmlC_Cupin.
 IPR007782; VKG_COase. 
Pfam
 PF05090; VKG_Carbox 
SMART
 SM00752; HTTM 
PROSITE
  
PRINTS