CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011347
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 5B 
Protein Synonyms/Alias
 eIF-5B; Translation initiation factor IF-2 
Gene Name
 Eif5b 
Gene Synonyms/Alias
 If2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
349AIQEALAKLKEEEERubiquitination[1]
762DRLYDWKKSPDSDVAubiquitination[1]
1011INIGPVHKKDVMKASubiquitination[1]
1091AVFPCKMKILPQYIFacetylation[2]
1181ATDILVSKISRQSIDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity). 
Sequence Annotation
 NP_BIND 634 641 GTP (By similarity).
 MOD_RES 108 108 Phosphoserine (By similarity).
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 137 137 Phosphoserine.
 MOD_RES 139 139 Phosphoserine.
 MOD_RES 165 165 Phosphoserine.
 MOD_RES 172 172 Phosphoserine (By similarity).
 MOD_RES 179 179 Phosphoserine.
 MOD_RES 183 183 Phosphoserine.
 MOD_RES 184 184 Phosphoserine.
 MOD_RES 187 187 Phosphoserine.
 MOD_RES 191 191 Phosphoserine.
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 300 300 Phosphothreonine (By similarity).
 MOD_RES 497 497 Phosphothreonine (By similarity).
 MOD_RES 556 556 Phosphoserine (By similarity).
 MOD_RES 584 584 Phosphoserine.
 MOD_RES 585 585 Phosphoserine.
 MOD_RES 587 587 Phosphoserine (By similarity).
 MOD_RES 591 591 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; GTP-binding; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1216 AA 
Protein Sequence
MGKKQKNKSE DSTKDDTDLG ALAAEIEGAG AAKEQEPQKS KGKKKKEKKK QDFDENDILR 60
ELEELSLEAQ GIRADRDAAA VKPTENNEEE SASKQDKKKK GQKGKKTSFD ENDSEELEDK 120
DSKSKKTARP NSEAPLSGSE DADDSNKLSK KGKKAQKSTK KRDGSEEDED NSKRSKERSR 180
VNSSGESGGE SDEFLQSRKG QKKNQKNKSV PTVDSGNEDD DSSFKIKTVA QKKAEKKERE 240
KKKRDEEKAK LRKMKEKEEL EKGKKEQSKQ REPQKRPEEE VLTLRGTPDT GAASEEKGDT 300
AAALEDDNEG DKKKKDKKKK KTEKDEKEKE KKKGPSKSTV KAIQEALAKL KEEEERQKRE 360
EEERIKRLEE LEAKRKEEER LEQEKRERKK QKEKERKERL KKEGKLLTKS QREARARAEV 420
TLRHLQAQGV EVPSKDSLPK KRPVYEDKKK KKTPQQLESK EVSETLEISA PVEAVDQGGP 480
EKEETPPSVE PEEEEDTEDA GLDDWEAMAS DEEREKEGNM IHIEVEENPE EEEEEEEEEE 540
EEESEDEEEE GDSEGSDGDE EDCKLSDEKD SGKAGDTKPS KDASSDSEYD SDDDRTKEER 600
AYDKAKRRIE KRRLEHGKNV NTEKLRAPII CVLGHVDTGK TKILDKLRHT HVQDGEAGGI 660
TQQIGATNVP LEAINEQTKM IKNFDRENVR IPGMLIIDTP GHESFSNLRN RGSSLCDIAI 720
LVVDIMHGLE PQTIESINIL KSKKCPFIVA LNKIDRLYDW KKSPDSDVAV TLKKQKKNTK 780
DEFEERAKAI IVEFAQQGLN AALFYENKDP RTFVSLVPTS AHTGDGMGSL IYLLVELTQT 840
MLSKRLAHCE ELRAQVMEVK ALPGMGTTID VILINGRLKE GDTIIVPGVE GPIVTQIRGL 900
LLPPPMKELR VKNQYEKHKE VEAAQGVKIL GKDLEKTLAG LPLLVAYKDD EIPVLKDELI 960
HELKQTLNAI KLEEKGVYVQ ASTLGSLEAL LEFLKTSEVP YAGINIGPVH KKDVMKASVM 1020
LEHDPQYAVI LAFDVRIERD AQEMADSLGV RIFSAEIIYH LFDAFTKYRQ DYKKQKQEEF 1080
KHIAVFPCKM KILPQYIFNS RDPIVIGVTV EAGQVKQGTP MCVPSKNFVD IGIVTSIEIN 1140
HKQVDVAKKG QEVCVKIEPI PGESPKMFGR HFEATDILVS KISRQSIDAL KDWFRDEMQK 1200
SDWQLIVELK KVFEII 1216 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0006184; P:GTP catabolic process; IEA:GOC. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR015760; TIF_IF2.
 IPR023115; TIF_IF2_dom3.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF11987; IF-2 
SMART
  
PROSITE
  
PRINTS
 PR00315; ELONGATNFCT.