UniProt Accession

 BLMH_MOUSE; Q8R016; Q3TJR8; Q8BLZ4; Q8BZH9; Q8C111; Q8CID9 

Genbank Protein ID

 AK035228; AK040799; AK051441; AK053872; AK150438; AK167325; AL603842; BC024090; BC027037; BC027362; BC027403 

Genbank Nucleotide ID

 BAC28989.1; BAC30706.1; BAC34639.1; BAC35568.1; BAE29560.1; BAE39427.1; CAI35070.1; AAH24090.1; AAH27037.1; AAH27362.1; AAH27403.1 

Protein Name

 Bleomycin hydrolase 

Protein Synonyms/Alias

 BH; BLM hydrolase; BMH 

Gene Name

 Blmh 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
297	SNMVGGRKTLYNNQP	ubiquitination	[1]
391	NQEGTFVKWRVENSW	acetylation	[2, 3, 4]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity (By similarity). 

Sequence Annotation

 ACT_SITE 73 73 By similarity.
 ACT_SITE 372 372 By similarity.
 ACT_SITE 396 396 By similarity.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 391 391 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Hydrolase; Protease; Reference proteome; Thiol protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 455 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
 GO:0008233; F:peptidase activity; IMP:MGI.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0042493; P:response to drug; IMP:MGI.
 GO:0009636; P:response to toxic substance; IMP:MGI. 

Interpro

 IPR000169; Pept_cys_AS.
 IPR004134; Peptidase_C1B. 

Pfam

 PF03051; Peptidase_C1_2 

SMART

  

PROSITE

 PS00139; THIOL_PROTEASE_CYS 

PRINTS