CPLM-000217

Genbank Nucleotide ID

Alpha-methylacyl-CoA racemase

Protein Synonyms/Alias

2-methylacyl-CoA racemase

Mus musculus (Mouse)

Position	Peptide	Type	References
57	RSLALDLKRSQGVTV	acetylation	[1, 2, 3, 4, 5]
57	RSLALDLKRSQGVTV	succinylation	[4]
86	FRCGVMEKLQLGPET	acetylation	[1, 2, 3, 4, 5]
86	FRCGVMEKLQLGPET	succinylation	[4]
100	TLLQDNPKLIYARLS	acetylation	[2, 3, 4, 5]
100	TLLQDNPKLIYARLS	succinylation	[4]
100	TLLQDNPKLIYARLS	ubiquitination	[6]
117	GQSGIFSKVAGHDIN	acetylation	[3, 4, 7]
117	GQSGIFSKVAGHDIN	succinylation	[4]
117	GQSGIFSKVAGHDIN	ubiquitination	[6]
134	ALSGVLSKIGRSGEN	ubiquitination	[6]
204	TQPMGLWKQPRGQNI	acetylation	[3, 4]
204	TQPMGLWKQPRGQNI	succinylation	[4]
204	TQPMGLWKQPRGQNI	ubiquitination	[6]
267	SADWPEMKKKFADVF	acetylation	[4]
267	SADWPEMKKKFADVF	succinylation	[4]
269	DWPEMKKKFADVFAK	acetylation	[4]
269	DWPEMKKKFADVFAK	succinylation	[4]
276	KFADVFAKKTKAEWC	acetylation	[2, 5]
340	TPAVPSAKRDPSVGE	acetylation	[3]
376	DRIVESDKLKANL**	acetylation	[2, 4, 5]
376	DRIVESDKLKANL**	succinylation	[4]

[1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
[2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[5] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[6] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[7] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]

Functional Description

Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.

MOTIF 379 381 Microbody targeting signal (By
ACT_SITE 151 151 Nucleophile (By similarity).

Complete proteome; Direct protein sequencing; Isomerase; Mitochondrion; Peroxisome; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005739; C:mitochondrion; IDA:MGI.
GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
GO:0008111; F:alpha-methylacyl-CoA racemase activity; TAS:MGI.
GO:0006699; P:bile acid biosynthetic process; IEA:UniProtKB-UniPathway.
GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-UniPathway.
GO:0008300; P:isoprenoid catabolic process; TAS:MGI.

IPR003673; CoA-Trfase_fam_III.
IPR023606; CoA-Trfase_III_dom.

PF02515; CoA_transf_3