CPLM-018260

Genbank Nucleotide ID

PHD finger protein 10

Protein Synonyms/Alias

BRG1-associated factor 45a; BAF45a; XAP135

Homo sapiens (Human)

Position	Peptide	Type	References
168	YSVILQEKERQRITD	ubiquitination	[1, 2, 3, 4]
206	YIKKAAKKAAEFNSN	ubiquitination	[2]
239	VIQVPQGKYKVLPTE	ubiquitination	[1, 3]
249	VLPTERTKVSSYPVA	ubiquitination	[1, 2, 3, 4, 5]
267	GQFQEYYKRYSPDEL	ubiquitination	[1, 3, 4]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron- specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).

ZN_FING 379 436 PHD-type 1; degenerate.
ZN_FING 438 481 PHD-type 2; degenerate.
REGION 89 295 SAY.
REGION 89 185 Essential to induce neural progenitor
REGION 292 334 Essential to induce neural progenitor
MOD_RES 2 2 N-acetylalanine.
MOD_RES 12 12 Phosphoserine.
MOD_RES 36 36 Phosphoserine.
MOD_RES 50 50 Phosphoserine.
MOD_RES 270 270 Phosphoserine.
MOD_RES 297 297 Phosphoserine.
MOD_RES 301 301 Phosphoserine.
MOD_RES 327 327 Phosphoserine.

Acetylation; Alternative splicing; Complete proteome; Metal-binding; Neurogenesis; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0071564; C:npBAF complex; ISS:UniProtKB.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.

IPR011011; Znf_FYVE_PHD.
IPR001965; Znf_PHD.
IPR019787; Znf_PHD-finger.
IPR013083; Znf_RING/FYVE/PHD.

PS01359; ZF_PHD_1
PS50016; ZF_PHD_2