UniProt Accession

 MDM4_HUMAN; O15151; Q2M2Y2; Q32SL2; Q6GS18; Q8IV83 

Genbank Protein ID

 AF007111; AY923176; AK223228; AY207458; AL512306; BC067299; BC105106 

Genbank Nucleotide ID

 AAB62928.1; AAY22054.1; BAD96948.1; AAO13494.1; CAI14100.1; AAH67299.1; AAI05107.1 

Protein Name

 Protein Mdm4 

Protein Synonyms/Alias

 Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4 

Gene Name

 MDM4 

Gene Synonyms/Alias

 MDMX 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
328	FRCWALRKDWYSDCS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. 

Sequence Annotation

 DOMAIN 26 106 SWIB.
 ZN_FING 300 329 RanBP2-type.
 ZN_FING 437 478 RING-type.
 REGION 246 332 Region II.
 REGION 393 490 Necessary for interaction with UBP2.
 MOTIF 442 445 Nuclear localization signal (Potential).
 MOD_RES 342 342 Phosphoserine; by CHEK2.
 MOD_RES 367 367 Phosphoserine; by CHEK1 and CHEK2.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 490 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; TAS:UniProtKB.
 GO:0008283; P:cell proliferation; IEP:UniProtKB.
 GO:0071456; P:cellular response to hypoxia; IEP:UniProtKB.
 GO:0030330; P:DNA damage response, signal transduction by p53 class mediator; IEP:UniProtKB.
 GO:0045023; P:G0 to G1 transition; IEP:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IEA:InterPro.
 GO:0071157; P:negative regulation of cell cycle arrest; IEA:InterPro.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0042177; P:negative regulation of protein catabolic process; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0006461; P:protein complex assembly; IDA:UniProtKB.
 GO:0050821; P:protein stabilization; IEP:UniProtKB. 

Interpro

 IPR015458; MDM4.
 IPR016495; p53_neg-reg_MDM_2/4.
 IPR003121; SWIB_MDM2_domain.
 IPR001876; Znf_RanBP2.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF02201; SWIB
 PF00641; zf-RanBP 

SMART

 SM00184; RING
 SM00547; ZnF_RBZ 

PROSITE

 PS01358; ZF_RANBP2_1
 PS50199; ZF_RANBP2_2
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS