CPLM-001322

Genbank Nucleotide ID

AN1-type zinc finger protein 5

Protein Synonyms/Alias

Zinc finger A20 domain-containing protein 2; Zinc finger protein 216

Homo sapiens (Human)

Position	Peptide	Type	References
35	GMCSVCYKEHLQRQQ	ubiquitination	[1]
86	AAGSTSEKSRNVPVA	ubiquitination	[1, 2, 3, 4, 5]
148	EKAPELPKPKKNRCF	ubiquitination	[2]
183	GLHRYSDKHNCPYDY	ubiquitination	[1, 4, 5]
191	HNCPYDYKAEAAAKI	ubiquitination	[1, 3, 4]
200	EAAAKIRKENPVVVA	ubiquitination	[1]
209	NPVVVAEKIQRI***	acetylation	[5, 6]
209	NPVVVAEKIQRI***	ubiquitination	[1, 5]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]

Functional Description

Involved in protein degradation via the ubiquitin- proteasome system. May act by anchoring ubiquitinated proteins to the proteasome. Plays a role in ubiquitin-mediated protein degradation during muscle atrophy. Plays a role in the regulation of NF-kappa-B activation and apoptosis. Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis factor (TNF), IL-1 and TLR4- induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Is a potent inhibitory factor for osteoclast differentiation.

ZN_FING 8 42 A20-type.
ZN_FING 151 194 AN1-type.
MOD_RES 48 48 Phosphoserine.

Complete proteome; Cytoplasm; Metal-binding; Phosphoprotein; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0003677; F:DNA binding; IEA:InterPro.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0060324; P:face development; IEA:Compara.
GO:0010761; P:fibroblast migration; IEA:Compara.
GO:0001701; P:in utero embryonic development; IEA:Compara.
GO:0048008; P:platelet-derived growth factor receptor signaling pathway; IEA:Compara.
GO:0003016; P:respiratory system process; IEA:Compara.
GO:0048705; P:skeletal system morphogenesis; IEA:Compara.
GO:0048745; P:smooth muscle tissue development; IEA:Compara.
GO:0001944; P:vasculature development; IEA:Compara.

IPR002653; Znf_A20.
IPR000058; Znf_AN1.

PF01754; zf-A20
PF01428; zf-AN1

SM00259; ZnF_A20
SM00154; ZnF_AN1

PS51036; ZF_A20
PS51039; ZF_AN1