UniProt Accession

 UBP18_HUMAN; Q9UMW8; Q6IAD9; Q9NY71 

Genbank Protein ID

 AF176642; AJ243526; AL136690; CR457216; BC014896 

Genbank Nucleotide ID

 AAD49967.1; CAB76398.1; CAB66625.1; CAG33497.1; AAH14896.1 

Protein Name

 Ubl carboxyl-terminal hydrolase 18 

Protein Synonyms/Alias

 43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; Ubl thioesterase 18 

Gene Name

 USP18 

Gene Synonyms/Alias

 ISG43 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
3	*****MSKAFGLLRQ	ubiquitination	[1]
173	ALYTIRVKDSLICVD	ubiquitination	[1]
204	SLFDVDSKPLKTLED	ubiquitination	[1, 2]
268	IRNSQTRKICHSLYF	ubiquitination	[1]
288	FSQILPMKRESCDAE	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. 

Sequence Annotation

 ACT_SITE 64 64 Nucleophile (By similarity).
 ACT_SITE 318 318 Proton acceptor (By similarity).  

Keyword

 Complete proteome; Hydrolase; Polymorphism; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 372 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:LIFEdb.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0004843; F:ubiquitin-specific protease activity; TAS:ProtInc.
 GO:0060338; P:regulation of type I interferon-mediated signaling pathway; TAS:Reactome.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 

Interpro

 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19. 

Pfam

 PF00443; UCH 

SMART

  

PROSITE

 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 

PRINTS