UniProt Accession

 HEM2_ECOLI; P0ACB2; P15002; P78247; Q2MC56 

Genbank Protein ID

 M24488; X17417; L44595; D85613; U73857; U00096; AP009048 

Genbank Nucleotide ID

 AAA62629.1; CAA35467.1; AAB52499.1; BAA12842.1; AAB18092.1; AAC73472.2; BAE76150.1 

Protein Name

 Delta-aminolevulinic acid dehydratase 

Protein Synonyms/Alias

 ALAD; ALADH; Porphobilinogen synthase 

Gene Name

 hemB 

Gene Synonyms/Alias

 ncf; b0369; JW0361 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
59	GVMRIPEKHLAREIE	acetylation	[1]
185	ALDAAGFKDTAIMSY	acetylation	[1]
195	AIMSYSTKFASSFYG	acetylation	[1]
217	SALKGDRKSYQMNPM	acetylation	[2]
247	GADCLMVKPAGAYLD	acetylation	[1]
292	AGAIDEEKVVLESLG	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 

Functional Description

 Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. 

Sequence Annotation

 ACT_SITE 195 195 Schiff-base intermediate with substrate.
 ACT_SITE 247 247 Schiff-base intermediate with substrate.
 METAL 120 120 Zinc; catalytic.
 METAL 122 122 Zinc; catalytic.
 METAL 130 130 Zinc; catalytic.
 METAL 232 232 Magnesium.
 BINDING 205 205 Substrate 1.
 BINDING 216 216 Substrate 1.
 BINDING 273 273 Substrate 2.
 BINDING 312 312 Substrate 2.  

Keyword

 3D-structure; Allosteric enzyme; Complete proteome; Direct protein sequencing; Heme biosynthesis; Lyase; Magnesium; Metal-binding; Porphyrin biosynthesis; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 324 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004655; F:porphobilinogen synthase activity; IMP:EcoliWiki.
 GO:0006783; P:heme biosynthetic process; IMP:EcoliWiki.
 GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR001731; Porphobilinogen_synth. 

Pfam

 PF00490; ALAD 

SMART

 SM01004; ALAD 

PROSITE

 PS00169; D_ALA_DEHYDRATASE 

PRINTS

 PR00144; DALDHYDRTASE.