CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011803
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sphingoid long chain base kinase 4 
Protein Synonyms/Alias
 LCB kinase 4; Sphinganine kinase 4 
Gene Name
 LCB4 
Gene Synonyms/Alias
 YOR171C; O3615 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
90KYVNVGQKGFNNGKIubiquitination[1]
125NHSLSNDKAPVSESQubiquitination[1]
143KKDKWDTKTNTVKVSubiquitination[1]
148DTKTNTVKVSPDDSQubiquitination[1, 2]
164NSPSLGIKDNQQLIEubiquitination[1]
441LENKDKNKGCLTFEPubiquitination[1]
469NNINNSTKDELSPNFubiquitination[1]
558PILLSLDKGSHVLEPubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Catalyzes the phosphorylation of the sphingoid long chain bases dihydrosphingosine (DHS or sphinganine) and phytosphingosine (PHS) to form dihydrosphingosine 1-phosphate (DHS-1P) and phytosphingosine 1-phosphate (PHS-1P) respectively. Involved in the biosynthesis of sphingolipids and ceramides. Required with LCB3 for an effective incorporation of DHS into ceramides through a phosphorylation-dephosphorylation cycle. Involved in heat-induced transient cell cycle arrest. Accumulation of phosphorylated sphingoid long chain bases (LCBPs) stimulates calcium influx and activates calcineurin signaling. Involved in heat-stress resistance. 
Sequence Annotation
 DOMAIN 224 363 DAGKc.
 MOD_RES 111 111 Phosphoserine.
 MOD_RES 120 120 Phosphoserine.
 MOD_RES 154 154 Phosphoserine.
 MOD_RES 160 160 Phosphoserine.
 MOD_RES 451 451 Phosphoserine; by PHO85.
 MOD_RES 454 454 Phosphoserine.
 MOD_RES 455 455 Phosphoserine; by PHO85.
 MOD_RES 460 460 Phosphoserine.
 LIPID 43 43 S-palmitoyl cysteine.
 LIPID 46 46 S-palmitoyl cysteine.  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Endoplasmic reticulum; Endosome; Golgi apparatus; Kinase; Lipid metabolism; Lipoprotein; Membrane; Nucleotide-binding; Palmitate; Phosphoprotein; Reference proteome; Sphingolipid metabolism; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 624 AA 
Protein Sequence
MVVQKKLRAI LTDEGVLIKS QSHHMFNKHG QLRSGDSLSL LSCLSCLDDG TLSSDGGSFD 60
EDDSLELLPL NTTIPFNRIL NAKYVNVGQK GFNNGKISSN PFQTENLSSS SENDDVENHS 120
LSNDKAPVSE SQSFPKKDKW DTKTNTVKVS PDDSQDNSPS LGIKDNQQLI ELTFAVPKGH 180
DVIPQKLTLL IDHVSRKSRA NTGEENISSG TVEEILEKSY ENSKRNRSIL VIINPHGGKG 240
TAKNLFLTKA RPILVESGCK IEIAYTKYAR HAIDIAKDLD ISKYDTIACA SGDGIPYEVI 300
NGLYRRPDRV DAFNKLAVTQ LPCGSGNAMS ISCHWTNNPS YAALCLVKSI ETRIDLMCCS 360
QPSYMNEWPR LSFLSQTYGV IAESDINTEF IRWMGPVRFN LGVAFNIIQG KKYPCEVFVK 420
YAAKSKKELK VHFLENKDKN KGCLTFEPNP SPNSSPDLLS KNNINNSTKD ELSPNFLNED 480
NFKLKYPMTE PVPRDWEKMD SELTDNLTIF YTGKMPYIAK DTKFFPAALP ADGTIDLVIT 540
DARIPVTRMT PILLSLDKGS HVLEPEVIHS KILAYKIIPK VESGLFSVDG EKFPLEPLQV 600
EIMPMLCKTL LRNGRYIDTE FESM 624 
Gene Ontology
 GO:0032541; C:cortical endoplasmic reticulum; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0017050; F:D-erythro-sphingosine kinase activity; IDA:SGD.
 GO:0004143; F:diacylglycerol kinase activity; IEA:InterPro.
 GO:0008481; F:sphinganine kinase activity; IEA:EC.
 GO:0019722; P:calcium-mediated signaling; IMP:SGD.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IEA:InterPro.
 GO:0006665; P:sphingolipid metabolic process; IDA:SGD. 
Interpro
 IPR001206; Diacylglycerol_kinase_cat_dom. 
Pfam
 PF00781; DAGK_cat 
SMART
 SM00046; DAGKc 
PROSITE
 PS50146; DAGK 
PRINTS