UniProt Accession

 VATE_YEAST; P22203; D6W328 

Genbank Protein ID

 M60663; Z49821; Z75239; BK006948 

Genbank Nucleotide ID

 AAA35209.1; CAA89978.1; CAA99654.1; DAA11094.1 

Protein Name

 V-type proton ATPase subunit E 

Protein Synonyms/Alias

 V-ATPase subunit E; V-ATPase 27 kDa subunit; Vacuolar proton pump subunit E 

Gene Name

 VMA4 

Gene Synonyms/Alias

 VAT5; YOR332W; O6241 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
38	KAKEIQLKADQEYEI	acetylation	[1]
47	DQEYEIEKTNIVRNE	acetylation	[1]
47	DQEYEIEKTNIVRNE	ubiquitination	[2]
63	NNIDGNFKSKLKKAM	acetylation	[1]
63	NNIDGNFKSKLKKAM	ubiquitination	[2]
68	NFKSKLKKAMLSQQI	ubiquitination	[2]
77	MLSQQITKSTIANKM	ubiquitination	[2]
87	IANKMRLKVLSAREQ	ubiquitination	[2]
140	LEPKAIVKALERDVD	acetylation	[1]
140	LEPKAIVKALERDVD	ubiquitination	[2]
153	VDLIESMKDDIMREY	acetylation	[1]
153	VDLIESMKDDIMREY	ubiquitination	[2]
208	NTLEERLKLLSEEAL	ubiquitination	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. 

Sequence Annotation

 REGION 8 20 Interaction with VMA5.
 REGION 19 38 Interaction with VMA10.
 MOD_RES 2 2 N-acetylserine.  

Keyword

 3D-structure; Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Hydrogen ion transport; Ion transport; Membrane; Reference proteome; Transport; Vacuole. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 233 AA 

Protein Sequence

Gene Ontology

 GO:0000329; C:fungal-type vacuole membrane; TAS:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0000221; C:vacuolar proton-transporting V-type ATPase, V1 domain; IDA:SGD.
 GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; TAS:SGD.
 GO:0015991; P:ATP hydrolysis coupled proton transport; IEA:InterPro.
 GO:0007035; P:vacuolar acidification; TAS:SGD. 

Interpro

 IPR002842; ATPase_V1/A1-cplx_esu. 

Pfam

 PF01991; vATP-synt_E 

SMART

  

PROSITE

  

PRINTS