CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007530
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ETS domain-containing protein Elk-3 
Protein Synonyms/Alias
 ETS-related protein ERP; ETS-related protein NET; Serum response factor accessory protein 2; SAP-2; SRF accessory protein 2 
Gene Name
 ELK3 
Gene Synonyms/Alias
 NET; SAP2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83IGQKFVYKFVSFPEIubiquitination[1]
92VSFPEILKMDPHAVEubiquitination[2]
113LLQDSDCKASPEGREubiquitination[1, 3]
162PDAFKAIKTEKLEEPsumoylation[4]
165FKAIKTEKLEEPPEDubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Sumoylation of the net inhibitory domain (NID) is stimulated by PIAS1 and has a negative effect on the transcriptional activity of Net.
 Wasylyk C, Criqui-Filipe P, Wasylyk B.
 Oncogene. 2005 Jan 27;24(5):820-8. [PMID: 15580297] 
Functional Description
 May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element. 
Sequence Annotation
 DNA_BIND 5 85 ETS.
 MOTIF 273 277 CTBP-binding motif.
 MOD_RES 115 115 Phosphoserine.  
Keyword
 Activator; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 407 AA 
Protein Sequence
MESAITLWQF LLQLLLDQKH EHLICWTSND GEFKLLKAEE VAKLWGLRKN KTNMNYDKLS 60
RALRYYYDKN IIKKVIGQKF VYKFVSFPEI LKMDPHAVEI SRESLLLQDS DCKASPEGRE 120
AHKHGLAALR STSRNEYIHS GLYSSFTINS LQNPPDAFKA IKTEKLEEPP EDSPPVEEVR 180
TVIRFVTNKT DKHVTRPVVS LPSTSEAAAA SAFLASSVSA KISSLMLPNA ASISSASPFS 240
SRSPSLSPNS PLPSEHRSLF LEAACHDSDS LEPLNLSSGS KTKSPSLPPK AKKPKGLEIS 300
APPLVLSGTD IGSIALNSPA LPSGSLTPAF FTAQTPNGLL LTPSPLLSSI HFWSSLSPVA 360
PLSPARLQGP STLFQFPTLL NGHMPVPIPS LDRAASPVLL SSNSQKS 407 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IC:HGNC.
 GO:0032422; F:purine-rich negative regulatory element binding; IDA:HGNC.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; IDA:HGNC.
 GO:0001525; P:angiogenesis; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:HGNC.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0042060; P:wound healing; IEA:Compara. 
Interpro
 IPR000418; Ets_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00178; Ets 
SMART
 SM00413; ETS 
PROSITE
 PS00345; ETS_DOMAIN_1
 PS00346; ETS_DOMAIN_2
 PS50061; ETS_DOMAIN_3 
PRINTS
 PR00454; ETSDOMAIN.