CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011616
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA(Thr) (cytosine(32)-N(3))-methyltransferase 
Protein Synonyms/Alias
 Actin-binding protein of 140 kDa; tRNA methyltransferase of 140 kDa 
Gene Name
 ABP140 
Gene Synonyms/Alias
 TRM140; YOR239W; YOR240W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
102SENKIYSKGENADINubiquitination[1]
476PRAVELVKNSEQFNPubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 S-adenosyl-L-methionine-dependent methyltransferase that mediates 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr) and tRNA(Ser). Binds F-actin and shows weak F-actin cross-linking activity. 
Sequence Annotation
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 150 150 Phosphothreonine.
 MOD_RES 321 321 Phosphoserine.
 MOD_RES 326 326 Phosphoserine.
 MOD_RES 347 347 Phosphothreonine.  
Keyword
 Actin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Methyltransferase; Phosphoprotein; Reference proteome; Ribosomal frameshifting; S-adenosyl-L-methionine; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 628 AA 
Protein Sequence
MGVADLIKKF ESISKEEGDA TVDTNSSSKP LKSNDETKEL HQQESTAVPQ EVDVNEEFEN 60
EPETINSSRT AEKPLETNLP KPETNEEDEE EGSMSENKIY SKGENADINV NDFQEYKEME 120
NTGAEVLASS VEESDAIQEG VAEETEGIAT PKQKENEKND ESEEESANNA SEPAEEYSQS 180
EEDADIEQSN GKETENAENA SQQANDGSTS TTTSKNKKKK NKKKNKKKRN GNVNTNANVD 240
DSTKTGENDD TTGDTTSSTT SAIQEVNDLE VVDDSCLGID QQHNREHLKA LTQDVKEETL 300
ENIAHEGRGD NTGDQNAVEK SDFEKSDTEG SRIGRDLPFE FGKRNLTEES DVWDHNAWDN 360
VEWGEEQVQQ AEEKIKEQFK HPVPEFDKKL YNENPARYWD IFYKNNKENF FKDRKWLQIE 420
FPILYASTRK DAEPVTIFEI GCGAGNTFFP ILKDNENENL RIIAADFAPR AVELVKNSEQ 480
FNPKYGHATV WDLANPDGNL PDGVEPHSVD IAVMIFVFSA LAPNQWDQAM DNLHKILKPG 540
GKIIFRDYGA YDLTQVRFKK NRILEENFYV RGDGTRVYFF SEEKLREIFT KKYFLENKIG 600
TDRRLLVNRK RQLKMYRCWV QAVFDVPQ 628 
Gene Ontology
 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0005884; C:actin filament; IDA:SGD.
 GO:0032432; C:actin filament bundle; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0051015; F:actin filament binding; IDA:SGD.
 GO:0030674; F:protein binding, bridging; IDA:SGD.
 GO:0052735; F:tRNA (cytosine-3-)-methyltransferase activity; IDA:SGD.
 GO:0051017; P:actin filament bundle assembly; IDA:SGD. 
Interpro
 IPR013217; Methyltransf_12.
 IPR026113; MeTrfase. 
Pfam
 PF08242; Methyltransf_12 
SMART
  
PROSITE
  
PRINTS