CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014662
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Calpain-3 
Protein Synonyms/Alias
 Calcium-activated neutral proteinase 3; CANP 3; Calpain L3; Calpain p94; Muscle-specific calcium-activated neutral protease 3 
Gene Name
 Capn3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
30VPHPAQGKTTEAGGGubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Calcium-regulated non-lysosomal thiol-protease. 
Sequence Annotation
 DOMAIN 74 417 Calpain catalytic.
 DOMAIN 649 683 EF-hand 1.
 DOMAIN 692 725 EF-hand 2.
 DOMAIN 722 757 EF-hand 3.
 DOMAIN 787 821 EF-hand 4.
 REGION 418 586 Domain III.
 REGION 587 649 Linker.
 REGION 650 820 Domain IV.
 ACT_SITE 129 129 By similarity.
 ACT_SITE 334 334 By similarity.
 ACT_SITE 358 358 By similarity.  
Keyword
 Alternative splicing; Calcium; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Protease; Reference proteome; Repeat; Thiol protease. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 821 AA 
Protein Sequence
MPTVISPTVA PRTGAEPRSP GPVPHPAQGK TTEAGGGHPS GIYSAIISRN FPIIGVKEKT 60
FEQLRRKCLE KKVLYLDPEF PPDETSLFYS QKFPIQFVWK RPPEICENPR FIIGGANRTD 120
ICQGDLGDCW FLAAIACLTL NERLLFRVIP HDQSFTENYA GIFHFQFWRY GDWVDVVIDD 180
CLPTYNNQLV FTKSNHRNEF WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVTEFFE 240
IKDAPSDMYK IMRKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLLR 300
DSDLDPRGSD DRPSRTIVPV QYETRMACGL VKGHAYSVTG LEEALFKGEK VKLVRLRNPW 360
GQVEWNGSWS DGWKDWSFVD KDEKARLQHQ VTEDGEFWMS YDDFVYHFTK LEICNLTADA 420
LESDKLQTWT VSVNEGRWVR GCSAGGCRNF PDTFWTNPQY RLKLLEEDDD PEDSEVICSF 480
LVALMQKNRR KDRKLGANLF TIGFAIYEVP KEMHGNKQHL QKDFFLYNAS KARSKTYINM 540
REVSQRFRLP PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEAENTISV DRPVKKKKNK 600
PIIFVSDRAN SNKELGVDQE AEEGKDKAGP EKRGETPQPR PGHTDQESEE QQQFRNIFRQ 660
IAGDDMEICA DELKNVLNTV VNKHKDLKTQ GFTLESCRSM IALMDTDGSG RLNLQEFHHL 720
WKKIKAWQKI FKHYDTDHSG TINSYEMRNA VNDAGFHLNS QLYDIITMRY ADKHMNIDFD 780
SFICCFVRLE GMFRAFNAFD KDGDGIIKLN VLEWLQLTMY A 821 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0043234; C:protein complex; IDA:UniProtKB.
 GO:0030315; C:T-tubule; ISS:UniProtKB.
 GO:0030018; C:Z disc; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IDA:MGI.
 GO:0055103; F:ligase regulator activity; ISS:UniProtKB.
 GO:0032947; F:protein complex scaffold; IMP:UniProtKB.
 GO:0031402; F:sodium ion binding; IDA:UniProtKB.
 GO:0008307; F:structural constituent of muscle; IMP:UniProtKB.
 GO:0031432; F:titin binding; ISS:UniProtKB.
 GO:0001896; P:autolysis; IDA:UniProtKB.
 GO:0071277; P:cellular response to calcium ion; IDA:UniProtKB.
 GO:0071472; P:cellular response to salt stress; IDA:UniProtKB.
 GO:0070315; P:G1 to G0 transition involved in cell differentiation; IDA:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
 GO:0033234; P:negative regulation of protein sumoylation; ISS:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; IMP:UniProtKB.
 GO:0045862; P:positive regulation of proteolysis; IDA:UniProtKB.
 GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; IMP:UniProtKB.
 GO:0014718; P:positive regulation of satellite cell activation involved in skeletal muscle regeneration; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0006461; P:protein complex assembly; IMP:UniProtKB.
 GO:0072657; P:protein localization to membrane; IMP:UniProtKB.
 GO:0043122; P:regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0045661; P:regulation of myoblast differentiation; IDA:UniProtKB.
 GO:0014850; P:response to muscle activity; ISS:UniProtKB.
 GO:0045214; P:sarcomere organization; IMP:MGI.
 GO:0097264; P:self proteolysis; ISS:UniProtKB. 
Interpro
 IPR022684; Calpain_cysteine_protease.
 IPR022682; Calpain_domain_III.
 IPR022683; Calpain_III.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000169; Pept_cys_AS.
 IPR001300; Peptidase_C2_calpain_cat. 
Pfam
 PF01067; Calpain_III
 PF13202; EF_hand_3
 PF13405; EF_hand_4
 PF00648; Peptidase_C2 
SMART
 SM00720; calpain_III
 SM00230; CysPc
 SM00054; EFh 
PROSITE
 PS50203; CALPAIN_CAT
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS00640; THIOL_PROTEASE_ASN
 PS00139; THIOL_PROTEASE_CYS
 PS00639; THIOL_PROTEASE_HIS 
PRINTS
 PR00704; CALPAIN.