UniProt Accession

 UXT_HUMAN; Q9UBK9; B2R561; Q5JZG3; Q9Y6E5 

Genbank Protein ID

 AF092737; AF083241; AF083242; AL009172; AL009172; AK312072; CH471164; BC000720; BC008890 

Genbank Nucleotide ID

 AAD28698.1; AAD39839.1; AAD39840.1; CAI42447.1; CAI42448.1; BAG35008.1; EAW59326.1; AAH00720.1; AAH08890.1 

Protein Name

 Protein UXT 

Protein Synonyms/Alias

 Androgen receptor trapped clone 27 protein; ART-27; Ubiquitously expressed transcript protein 

Gene Name

 UXT 

Gene Synonyms/Alias

 HSPC024 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
16	AVEATGEKVLRYETF	ubiquitination	[1, 2, 3, 4, 5]
41	KVLDHRDKVYEQLAK	ubiquitination	[1, 3, 4, 6]
48	KVYEQLAKYLQLRNV	ubiquitination	[1, 4, 6, 7, 8, 9]
113	ALKFIDRKSSLLTEL	ubiquitination	[1, 6, 9, 10, 11]
126	ELSNSLTKDSMNIKA	ubiquitination	[1, 2, 4, 5, 6, 7, 9]
154	GLQNFPEKPHH****	ubiquitination	[4, 7, 9]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [10] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [11] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor transcription (AR). AR N-terminus-associated coactivator which may play a role in facilitating receptor-induced transcriptional activation (PubMed:11854421). Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling (PubMed:11827465). Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC (PubMed:17554592). May be a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function (PubMed:17620405). Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM (PubMed:17635584). Together with URI1, associates with chromatin to the NKX3-1 promoter region. 

Sequence Annotation

  

Keyword

 Activator; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 157 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:HGNC.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0016272; C:prefoldin complex; IEA:InterPro.
 GO:0048487; F:beta-tubulin binding; IDA:HGNC.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0008017; F:microtubule binding; TAS:HGNC.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IDA:UniProtKB.
 GO:0051297; P:centrosome organization; IMP:HGNC.
 GO:0047497; P:mitochondrion transport along microtubule; TAS:HGNC.
 GO:0006457; P:protein folding; IEA:InterPro. 

Interpro

 IPR003994; PFD_UXT.
 IPR009053; Prefoldin.
 IPR004127; Prefoldin_subunit. 

Pfam

 PF02996; Prefoldin 

SMART

  

PROSITE

  

PRINTS

 PR01502; UXTPROTEIN.