UniProt Accession

 CYO2_VIOOD; P58434 

Genbank Protein ID

  

Genbank Nucleotide ID

  

Protein Name

 Cycloviolacin-O2 

Protein Synonyms/Alias

  

Gene Name

  

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Viola odorata (Sweet violet) 

NCBI Taxa ID

 97441 

Lysine Modification

Position	Peptide	Type	References
23	SAIGCSCKSKVCYRN	acetylation	[1, 2]
25	IGCSCKSKVCYRN**	acetylation	[1, 2]

Reference

 [1] Key role of glutamic acid for the cytotoxic activity of the cyclotide cycloviolacin O2.
 Herrmann A, Svangård E, Claeson P, Gullbo J, Bohlin L, Göransson U.
 Cell Mol Life Sci. 2006 Jan;63(2):235-45. [PMID: 16389447]
 [2] The anthelmintic activity of the cyclotides: natural variants with enhanced activity.
 Colgrave ML, Kotze AC, Ireland DC, Wang CK, Craik DJ.
 Chembiochem. 2008 Aug 11;9(12):1939-45. [PMID: 18618891] 

Functional Description

 Probably participates in a plant defense mechanism. Has strong cytotoxic activity against a variety of drug-resistant and drug-sensitive human tumor cell lines, and against primary chronic lymphocytic leukemia and ovarian carcinoma cells. Has weaker cytotoxic activity against normal lymphocytes. Has hemolytic activity. 

Sequence Annotation

 DISULFID 4 20
 DISULFID 8 22
 DISULFID 13 27
 CROSSLNK 1 30 Cyclopeptide (Gly-Asn).  

Keyword

 3D-structure; Cytolysis; Direct protein sequencing; Disulfide bond; Hemolysis; Knottin; Plant defense. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 30 AA 

Protein Sequence

Gene Ontology

 GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
 GO:0006952; P:defense response; IDA:UniProtKB.
 GO:0044179; P:hemolysis in other organism; IEA:UniProtKB-KW. 

Interpro

 IPR005535; Cyclotide.
 IPR012323; Cyclotide_bracelet_CS.
 IPR017307; Cyclotide_subgr. 

Pfam

 PF03784; Cyclotide 

SMART

  

PROSITE

 PS51052; CYCLOTIDE
 PS60008; CYCLOTIDE_BRACELET 

PRINTS