UniProt Accession

 CHP3_HUMAN; Q96BS2; F5H1Y5; Q9NWT9 

Genbank Protein ID

 AF443207; AK000614; AC026368; CH471054; BC015221; BQ949772 

Genbank Nucleotide ID

 AAL35615.1; BAA91288.1; EAW98101.1; AAH15221.1 

Protein Name

 Calcineurin B homologous protein 3 

Protein Synonyms/Alias

 Tescalcin; TSC 

Gene Name

 TESC 

Gene Synonyms/Alias

 CHP3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
33	EQLHRRFKQLSGDQP	ubiquitination	[1, 2, 3]
44	GDQPTIRKENFNNVP	ubiquitination	[2]
151	SGNPHIEKESARSIA	ubiquitination	[2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Promotes the maturation, transport, cell surface stability and exchange activity of SLC9A1/NHE1 at the plasma membrane. Promotes the induction of hematopoietic stem cell differentiation toward megakaryocytic lineage. Essential for the coupling of ERK cascade activation with the expression of ETS family genes in megakaryocytic differentiation. Also involved in granulocytic differentiation in a ERK-dependent manner. Inhibits the phosphatase activity of calcineurin. 

Sequence Annotation

 DOMAIN 110 145 EF-hand.
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 Alternative splicing; Calcium; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Differentiation; Disulfide bond; Lipoprotein; Membrane; Metal-binding; Myristate; Nucleus; Protein kinase inhibitor; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 214 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0001726; C:ruffle; ISS:UniProtKB.
 GO:0032587; C:ruffle membrane; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0019212; F:phosphatase inhibitor activity; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0004860; F:protein kinase inhibitor activity; IEA:UniProtKB-KW.
 GO:0071300; P:cellular response to retinoic acid; IDA:UniProtKB.
 GO:0008584; P:male gonad development; IEA:Compara.
 GO:0030219; P:megakaryocyte differentiation; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
 GO:0006469; P:negative regulation of protein kinase activity; IEA:GOC.
 GO:0030854; P:positive regulation of granulocyte differentiation; IDA:UniProtKB.
 GO:0045654; P:positive regulation of megakaryocyte differentiation; IMP:UniProtKB.
 GO:0032417; P:positive regulation of sodium:hydrogen antiporter activity; IDA:UniProtKB.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IMP:UniProtKB.
 GO:0051604; P:protein maturation; IDA:UniProtKB.
 GO:0050821; P:protein stabilization; IDA:UniProtKB.
 GO:0072661; P:protein targeting to plasma membrane; IDA:UniProtKB.
 GO:0033628; P:regulation of cell adhesion mediated by integrin; IMP:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 

Pfam

 PF13405; EF_hand_4 

SMART

  

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS