CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020338
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Oxysterol-binding protein-related protein 1 
Protein Synonyms/Alias
 ORP-1; OSBP-related protein 1 
Gene Name
 OSBPL1A 
Gene Synonyms/Alias
 ORP1; OSBP8; OSBPL1; OSBPL1B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
365DLKKSLEKAQSCQQRubiquitination[1]
623SQWERTGKPFNPLLGubiquitination[2]
682PKLKFWGKSVEAEPKubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3- phosphate (By similarity). Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A. 
Sequence Annotation
 REPEAT 47 76 ANK 1.
 REPEAT 80 109 ANK 2.
 REPEAT 175 204 ANK 3.
 DOMAIN 235 334 PH.
 REGION 1 237 Interaction with RAB7A.
 MOD_RES 499 499 Phosphoserine.  
Keyword
 Alternative splicing; ANK repeat; Coiled coil; Complete proteome; Endosome; Lipid transport; Lipid-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 950 AA 
Protein Sequence
MNTEAEQQLL HHARNGNAEE VRQLLETMAR NEVIADINCK GRSKSNLGWT PLHLACYFGH 60
RQVVQDLLKA GAEVNVLNDM GDTPLHRAAF TGRKELVMLL LEYNADTTIV NGSGQTAKEV 120
THAEEIRSML EAVERTQQRK LEELLLAAAR EGKTTELTAL LNRPNPPDVN CSDQLGNTPL 180
HCAAYRAHKQ CALKLLRSGA DPNLKNKNDQ KPLDLAQGAE MKHILVGNKV IYKALKRYEG 240
PLWKSSRFFG WRLFWVVLEH GVLSWYRKQP DAVHNIYRQG CKHLTQAVCT VKSTDSCLFF 300
IKCFDDTIHG FRVPKNSLQQ SREDWLEAIE EHSAYSTHYC SQDQLTDEEE EDTVSAADLK 360
KSLEKAQSCQ QRLDREISNF LKMIKECDMA KEMLPSFLQK VEVVSEASRE TCVALTDCLN 420
LFTKQEGVRN FKLEQEQEKN KILSEALETL ATEHHELEQS LVKGSPPASI LSEDEFYDAL 480
SDSESERSLS RLEAVTARSF EEEGEHLGSR KHRMSEEKDC GGGDALSNGI KKHRTSLPSP 540
MFSRNDFSIW SILRKCIGME LSKITMPVIF NEPLSFLQRL TEYMEHTYLI HKASSLSDPV 600
ERMQCVAAFA VSAVASQWER TGKPFNPLLG ETYELVRDDL GFRLISEQVS HHPPISAFHA 660
EGLNNDFIFH GSIYPKLKFW GKSVEAEPKG TITLELLEHN EAYTWTNPTC CVHNIIVGKL 720
WIEQYGNVEI INHKTGDKCV LNFKPCGLFG KELHKVEGYI QDKSKKKLCA LYGKWTECLY 780
SVDPATFDAY KKNDKKNTEE KKNSKQMSTS EELDEMPVPD SESVFIIPGS VLLWRIAPRP 840
PNSAQMYNFT SFAMVLNEVD KDMESVIPKT DCRLRPDIRA MENGEIDQAS EEKKRLEEKQ 900
RAARKNRSKS EEDWKTRWFH QGPNPYNGAQ DWIYSGSYWD RNYFNLPDIY 950 
Gene Ontology
 GO:0005770; C:late endosome; IDA:UniProtKB.
 GO:0005765; C:lysosomal membrane; TAS:Reactome.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0015485; F:cholesterol binding; IDA:BHF-UCL.
 GO:0005543; F:phospholipid binding; NAS:UniProtKB.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0008203; P:cholesterol metabolic process; NAS:UniProtKB.
 GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
 GO:0016192; P:vesicle-mediated transport; NAS:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR000648; Oxysterol-bd.
 IPR018494; Oxysterol-bd_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF12796; Ank_2
 PF01237; Oxysterol_BP 
SMART
 SM00248; ANK
 SM00233; PH 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS01013; OSBP
 PS50003; PH_DOMAIN 
PRINTS
 PR01415; ANKYRIN.