UniProt Accession

 SUS1_YEAST; Q6WNK7; D6VQB1 

Genbank Protein ID

 AY278445; Z35980; Z35981; BK006936 

Genbank Nucleotide ID

 AAQ19492.1; DAA07231.1 

Protein Name

 Protein SUS1 

Protein Synonyms/Alias

  

Gene Name

 SUS1 

Gene Synonyms/Alias

 YBR111W-A 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
30	ELISNELKARLLQEG	ubiquitination	[1]
68	ILSTVEPKALEMVSD	ubiquitination	[2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Involved in mRNA export coupled transcription activation by association with both the TREX-2 and the SAGA complexes. The transcription regulatory histone acetylation (HAT) complex SAGA is involved in RNA polymerase II-dependent regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SUS1 forms a distinct functional SAGA module with UBP8, SGF11 and SGF73 required for deubiquitination of H2B and for the maintenance of steady-state H3 methylation levels. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. SUS1 has also a role in mRNP biogenesis and maintenance of genome integrity through preventing RNA-mediated genome instability. Finally SUS1 has a role in response to DNA damage induced by methyl methane sulfonate (MMS) and replication arrest induced by hydroxyurea. 

Sequence Annotation

  

Keyword

 3D-structure; Activator; Chromatin regulator; Complete proteome; mRNA transport; Nuclear pore complex; Nucleus; Protein transport; Reference proteome; Transcription; Transcription regulation; Translocation; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 96 AA 

Protein Sequence

Gene Ontology

 GO:0071819; C:DUBm complex; IDA:SGD.
 GO:0005643; C:nuclear pore; IDA:SGD.
 GO:0000124; C:SAGA complex; IDA:SGD.
 GO:0070390; C:transcription export complex 2; IDA:SGD.
 GO:0008047; F:enzyme activator activity; IDA:SGD.
 GO:0016578; P:histone deubiquitination; IMP:SGD.
 GO:0051568; P:histone H3-K4 methylation; IMP:SGD.
 GO:0034729; P:histone H3-K79 methylation; IMP:SGD.
 GO:0071033; P:nuclear retention of pre-mRNA at the site of transcription; IMP:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IPI:SGD.
 GO:0000973; P:posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0032880; P:regulation of protein localization; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD. 

Interpro

 IPR018783; TF_enhancer_of_yellow_2. 

Pfam

 PF10163; EnY2 

SMART

  

PROSITE

  

PRINTS