| Tag | Content |
|---|
| CPLM ID | CPLM-005407 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Aconitate hydratase 1 |
Protein Synonyms/Alias | Aconitase 1; Citrate hydro-lyase 1 |
Gene Name | acnA |
Gene Synonyms/Alias | acn; b1276; JW1268 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 18 | DTLQAKDKTYHYYSL | acetylation | [1, 2] | | 30 | YSLPLAAKSLGDITR | acetylation | [2] | | 71 | HALAGWLKNAHADRE | acetylation | [1] | | 161 | HERYVFLKWGKQAFS | acetylation | [2] | | 164 | YVFLKWGKQAFSRFS | acetylation | [2] | | 283 | RKHGVVGKFVEFYGD | acetylation | [2] | | 342 | DQVELVEKYAKAQGM | acetylation | [2] | | 391 | VALPDVPKAFAASNE | acetylation | [1, 2] | | 460 | KAVTLGLKRQPWVKA | acetylation | [2] | | 482 | VVSDYLAKAKLTPYL | acetylation | [1, 2] | | 585 | KGDPVYLKDIWPSAQ | acetylation | [2] | | 684 | ISPAGSIKPDSPAGR | acetylation | [1, 2] | | 758 | YDAAMRYKQEQTPLA | acetylation | [1] | | 770 | PLAVIAGKEYGSGSS | acetylation | [2] | | 832 | LGLTGEEKIDIGDLQ | acetylation | [2] |
|
Reference | [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli. Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z. J Proteome Res. 2013 Feb 1;12(2):844-51. [ PMID: 23294111] [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the isomerization of citrate to isocitrate via cis-aconitate. May have an iron-responsive regulatory function. |
Sequence Annotation | METAL 435 435 Iron-sulfur (4Fe-4S) (By similarity).
METAL 501 501 Iron-sulfur (4Fe-4S) (By similarity).
METAL 504 504 Iron-sulfur (4Fe-4S) (By similarity). |
Keyword | 4Fe-4S; Complete proteome; Direct protein sequencing; Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome; Tricarboxylic acid cycle. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 891 AA |
Protein Sequence | MSSTLREASK DTLQAKDKTY HYYSLPLAAK SLGDITRLPK SLKVLLENLL RWQDGNSVTE 60
EDIHALAGWL KNAHADREIA YRPARVLMQD FTGVPAVVDL AAMREAVKRL GGDTAKVNPL 120
SPVDLVIDHS VTVDRFGDDE AFEENVRLEM ERNHERYVFL KWGKQAFSRF SVVPPGTGIC 180
HQVNLEYLGK AVWSELQDGE WIAYPDTLVG TDSHTTMING LGVLGWGVGG IEAEAAMLGQ 240
PVSMLIPDVV GFKLTGKLRE GITATDLVLT VTQMLRKHGV VGKFVEFYGD GLDSLPLADR 300
ATIANMSPEY GATCGFFPID AVTLDYMRLS GRSEDQVELV EKYAKAQGMW RNPGDEPIFT 360
STLELDMNDV EASLAGPKRP QDRVALPDVP KAFAASNELE VNATHKDRQP VDYVMNGHQY 420
QLPDGAVVIA AITSCTNTSN PSVLMAAGLL AKKAVTLGLK RQPWVKASLA PGSKVVSDYL 480
AKAKLTPYLD ELGFNLVGYG CTTCIGNSGP LPDPIETAIK KSDLTVGAVL SGNRNFEGRI 540
HPLVKTNWLA SPPLVVAYAL AGNMNINLAS EPIGHDRKGD PVYLKDIWPS AQEIARAVEQ 600
VSTEMFRKEY AEVFEGTAEW KGINVTRSDT YGWQEDSTYI RLSPFFDEMQ ATPAPVEDIH 660
GARILAMLGD SVTTDHISPA GSIKPDSPAG RYLQGRGVER KDFNSYGSRR GNHEVMMRGT 720
FANIRIRNEM VPGVEGGMTR HLPDSDVVSI YDAAMRYKQE QTPLAVIAGK EYGSGSSRDW 780
AAKGPRLLGI RVVIAESFER IHRSNLIGMG ILPLEFPQGV TRKTLGLTGE EKIDIGDLQN 840
LQPGATVPVT LTRADGSQEV VPCRCRIDTA TELTYYQNDG ILHYVIRNML K 891 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:EcoliWiki. GO:0051539; F:4 iron, 4 sulfur cluster binding; IDA:EcoCyc. GO:0003994; F:aconitate hydratase activity; IDA:EcoCyc. GO:0052632; F:citrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC. GO:0005506; F:iron ion binding; IMP:EcoliWiki. GO:0052633; F:isocitrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC. GO:0003730; F:mRNA 3'-UTR binding; IDA:EcoCyc. GO:0009061; P:anaerobic respiration; IDA:EcoliWiki. GO:0006097; P:glyoxylate cycle; NAS:EcoliWiki. GO:0006979; P:response to oxidative stress; IDA:EcoliWiki. GO:0006099; P:tricarboxylic acid cycle; NAS:EcoliWiki. |
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