UniProt Accession

 GLYAT_MOUSE; Q91XE0; Q05DG2 

Genbank Protein ID

 AK039262; AK143901; AK143803; BC010799; BC015294; BC024434 

Genbank Nucleotide ID

 BAC30297.1; BAE25590.1; BAE25544.1; AAH10799.1; AAH15294.1; AAH24434.1 

Protein Name

 Glycine N-acyltransferase 

Protein Synonyms/Alias

 Acyl-CoA:glycine N-acyltransferase; AAc; Aralkyl acyl-CoA N-acyltransferase; Aralkyl acyl-CoA:amino acid N-acyltransferase; Benzoyl-coenzyme A:glycine N-acyltransferase; Glycine N-benzoyltransferase 

Gene Name

 Glyat 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
16	QMLQMLEKSLRKYLP	acetylation	[1, 2, 3, 4, 5, 6]
16	QMLQMLEKSLRKYLP	succinylation	[5]
20	MLEKSLRKYLPESLK	acetylation	[2]
113	SSQSHLNKTIQNLAS	acetylation	[4, 6]
127	SIQSFQIKHSENILY	acetylation	[4, 5, 6]
127	SIQSFQIKHSENILY	succinylation	[5]
141	YVSSETIKKLFPSLL	acetylation	[4, 5, 6]
141	YVSSETIKKLFPSLL	succinylation	[5]
141	YVSSETIKKLFPSLL	ubiquitination	[7]
142	VSSETIKKLFPSLLD	acetylation	[4, 5]
142	VSSETIKKLFPSLLD	succinylation	[5]
142	VSSETIKKLFPSLLD	ubiquitination	[7]
151	FPSLLDTKNLSTGSG	acetylation	[4]
151	FPSLLDTKNLSTGSG	ubiquitination	[7]
159	NLSTGSGKPKAIDQD	acetylation	[2, 4, 5, 6]
159	NLSTGSGKPKAIDQD	succinylation	[5]
161	STGSGKPKAIDQDKF	acetylation	[5]
161	STGSGKPKAIDQDKF	succinylation	[5]
167	PKAIDQDKFKLSSLD	acetylation	[4, 5, 6]
167	PKAIDQDKFKLSSLD	succinylation	[5]
167	PKAIDQDKFKLSSLD	ubiquitination	[7]
169	AIDQDKFKLSSLDVV	acetylation	[4, 5, 6]
169	AIDQDKFKLSSLDVV	succinylation	[5]
169	AIDQDKFKLSSLDVV	ubiquitination	[7]
183	VHAALVNKFWLFGGN	acetylation	[4, 5]
183	VHAALVNKFWLFGGN	succinylation	[5]
256	SQDQIMTKRGYPVYS	acetylation	[4, 5]
256	SQDQIMTKRGYPVYS	succinylation	[5]
267	PVYSHTEKSNIAMQK	acetylation	[4, 5, 6]
267	PVYSHTEKSNIAMQK	succinylation	[5]

Reference

 [1] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [2] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [7] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate a multitude of substrates to form a variety of N-acylglycines, thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid (By similarity). 

Sequence Annotation

 MOD_RES 20 20 N6-acetyllysine.  

Keyword

 Acetylation; Acyltransferase; Alternative splicing; Complete proteome; Detoxification; Mitochondrion; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 296 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0047961; F:glycine N-acyltransferase activity; IEA:EC.
 GO:0047962; F:glycine N-benzoyltransferase activity; ISS:UniProtKB.
 GO:0006544; P:glycine metabolic process; ISS:UniProtKB.
 GO:0032787; P:monocarboxylic acid metabolic process; ISS:UniProtKB.
 GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW. 

Interpro

 IPR016181; Acyl_CoA_acyltransferase.
 IPR010313; Glycine_N-acyltransferase.
 IPR013652; Glycine_N-acyltransferase_C.
 IPR015938; Glycine_N-acyltransferase_N. 

Pfam

 PF08444; Gly_acyl_tr_C
 PF06021; Gly_acyl_tr_N 

SMART

  

PROSITE

  

PRINTS