CPLM-009601

Genbank Nucleotide ID

DNA-directed RNA polymerases I, II, and III subunit RPABC5

Protein Synonyms/Alias

RNA polymerases I, II, and III subunit ABC5; DNA-directed RNA polymerase III subunit L; RNA polymerase II 7.6 kDa subunit; RPB7.6; RPB10 homolog

Homo sapiens (Human)

Position	Peptide	Type	References
12	VRCFTCGKIVGNKWE	ubiquitination	[1, 2]
41	ALDALGLKRYCCRRM	ubiquitination	[3, 4]
58	AHVDLIEKLLNYAPL	ubiquitination	[3, 4, 5]
67	LNYAPLEK*******	ubiquitination	[1, 2, 3]

[1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2L/RBP10 is part of the core element with the central large cleft (By similarity).

METAL 7 7 Zinc (By similarity).
METAL 10 10 Zinc (By similarity).
METAL 44 44 Zinc (By similarity).
METAL 45 45 Zinc (By similarity).

Complete proteome; DNA-directed RNA polymerase; Metal-binding; Nucleus; Reference proteome; Transcription; Zinc.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005665; C:DNA-directed RNA polymerase II, core complex; IDA:UniProtKB.
GO:0003677; F:DNA binding; IEA:InterPro.
GO:0003899; F:DNA-directed RNA polymerase activity; TAS:ProtInc.
GO:0008270; F:zinc ion binding; IEA:InterPro.
GO:0006370; P:7-methylguanosine mRNA capping; TAS:Reactome.
GO:0000398; P:mRNA splicing, via spliceosome; TAS:Reactome.
GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
GO:0006356; P:regulation of transcription from RNA polymerase I promoter; TAS:ProtInc.
GO:0006386; P:termination of RNA polymerase III transcription; TAS:Reactome.
GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
GO:0006385; P:transcription elongation from RNA polymerase III promoter; TAS:Reactome.
GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome.
GO:0006283; P:transcription-coupled nucleotide-excision repair; TAS:Reactome.
GO:0022415; P:viral reproductive process; TAS:Reactome.

IPR009057; Homeodomain-like.
IPR023580; RNA_pol_su_RPB10.
IPR020789; RNA_pol_suN_Zn-BS.
IPR000268; RNAP_N/Rpb10.

PS01112; RNA_POL_N_8KD