CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020262
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup85 
Protein Synonyms/Alias
 85 kDa nucleoporin; FROUNT; Nucleoporin Nup75; Nucleoporin Nup85; Pericentrin-1 
Gene Name
 NUP85 
Gene Synonyms/Alias
 NUP75; PCNT1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
20IPGVNSKKNQMYFDWubiquitination[1]
44TSFNKKEKSEMVPSCubiquitination[1]
59PFIYIIRKDVDVYSQubiquitination[1, 2, 3, 4, 5]
70VYSQILRKLFNESHGubiquitination[1, 2, 3, 5, 6]
92IDEELTGKSRKSQLVubiquitination[1, 3, 6, 7]
124HQVAIAAKDPANGRQubiquitination[1, 8]
218EARQMLSKEADASPAubiquitination[1, 8]
257TLTELELKWQHWHEEubiquitination[1]
449IPLNTEQKALKVLRIubiquitination[1, 3, 6, 7, 8]
452NTEQKALKVLRICEQubiquitination[1]
471EQVRSICKILAMKAVubiquitination[1]
476ICKILAMKAVRNNRLubiquitination[1, 6]
494LSWSIRAKDAAFATLubiquitination[1, 3, 6]
540DRLTFLGKYREFHRMubiquitination[1, 2, 4, 5, 6]
631DDDIETTKVEMLRLSubiquitination[1, 3, 6, 8]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 223 223 Phosphoserine.  
Keyword
 Acetylation; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Kinetochore; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 656 AA 
Protein Sequence
MEELDGEPTV TLIPGVNSKK NQMYFDWGPG EMLVCETSFN KKEKSEMVPS CPFIYIIRKD 60
VDVYSQILRK LFNESHGIFL GLQRIDEELT GKSRKSQLVR VSKNYRSVIR ACMEEMHQVA 120
IAAKDPANGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL 180
SADVLGSENP SKHDSFWNLV TILVLQGRLD EARQMLSKEA DASPASAGIC RIMGDLMRTM 240
PILSPGNTQT LTELELKWQH WHEECERYLQ DSTFATSPHL ESLLKIMLGD EAALLEQKEL 300
LSNWYHFLVT RLLYSNPTVK PIDLHYYAQS SLDLFLGGES SPEPLDNILL AAFEFDIHQV 360
IKECSIALSN WWFVAHLTDL LDHCKLLQSH NLYFGSNMRE FLLLEYASGL FAHPSLWQLG 420
VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVRSIC KILAMKAVRN 480
NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGPAMM LSDRLTFLGK 540
YREFHRMYGE KRFADAASLL LSLMTSRIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL 600
MRCLEDLTSR RPVHGESDTE QLQDDDIETT KVEMLRLSLA RNLARAIIRE GSLEGS 656 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0031080; C:nuclear pore outer ring; IDA:UniProtKB.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0030032; P:lamellipodium assembly; IEA:Compara.
 GO:0048246; P:macrophage chemotaxis; IEA:Compara.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR011502; Nucleoporin_Nup85. 
Pfam
 PF07575; Nucleopor_Nup85 
SMART
  
PROSITE
  
PRINTS