CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000135
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein argonaute 1 
Protein Synonyms/Alias
  
Gene Name
 AGO1 
Gene Synonyms/Alias
 At1g48410; F11A17.3; T1N15.2 
Created Date
 July 27, 2013 
Organism
 Arabidopsis thaliana (Mouse-ear cress) 
NCBI Taxa ID
 3702 
Lysine Modification
Position
Peptide
Type
References
837FRRSTGHKPLRIIFYacetylation[1]
Reference
 [1] Lysine acetylation is a widespread protein modification for diverse proteins in Arabidopsis.
 Wu X, Oh MH, Schwarz EM, Larue CT, Sivaguru M, Imai BS, Yau PM, Ort DR, Huber SC.
 Plant Physiol. 2011 Apr;155(4):1769-78. [PMID: 21311030] 
Functional Description
 Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Requires DRB1 for directional loading of the small RNA duplex (guide stand and passenger strand) onto RISC for passenger strand degradation. Unlike animal RISC that associates in high molecular weight complex, Arabidopsis RISC is probably composed only of the AGO1 protein and associated RNA without any other proteins. Associates mainly with miRNAs of 21 nucleotide in length and preferentially recruits small RNAs with a 5' terminal uridine. Associates with 22 nucleotide miRNAs to trigger RDR6-dependent secondary siRNAs biogenesis. This pathway amplifies silencing by using the target RNA as substrate to generate secondary siRNAs. Binds to miR168 which targets its own mRNA for repression, establishing a homeostatic regulatory loop. Involved in antiviral RNA silencing by contributing to viral RNA clearance. Is capable of targeting viral RNAs with more compact structures than AGO7 which favors less structured RNA targets. May not associate with 24 nucleotide siRNAs involved in chromatin silencing. Essential for multiple processes in development. Essential for proper development of leaves and floral organs, and formation of axillary meristems. Like AGO10, required for stem cell function and organ polarity. 
Sequence Annotation
 DOMAIN 391 501 PAZ.
 DOMAIN 676 997 Piwi.
 MOD_RES 1001 1001 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Developmental protein; Host-virus interaction; Phosphoprotein; Plant defense; Reference proteome; Repressor; Ribonucleoprotein; RNA-binding; RNA-mediated gene silencing; Transcription; Transcription regulation; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1048 AA 
Protein Sequence
MVRKRRTDAP SEGGEGSGSR EAGPVSGGGR GSQRGGFQQG GGQHQGGRGY TPQPQQGGRG 60
GRGYGQPPQQ QQQYGGPQEY QGRGRGGPPH QGGRGGYGGG RGGGPSSGPP QRQSVPELHQ 120
ATSPTYQAVS SQPTLSEVSP TQVPEPTVLA QQFEQLSVEQ GAPSQAIQPI PSSSKAFKFP 180
MRPGKGQSGK RCIVKANHFF AELPDKDLHH YDVTITPEVT SRGVNRAVMK QLVDNYRDSH 240
LGSRLPAYDG RKSLYTAGPL PFNSKEFRIN LLDEEVGAGG QRREREFKVV IKLVARADLH 300
HLGMFLEGKQ SDAPQEALQV LDIVLRELPT SRYIPVGRSF YSPDIGKKQS LGDGLESWRG 360
FYQSIRPTQM GLSLNIDMSS TAFIEANPVI QFVCDLLNRD ISSRPLSDAD RVKIKKALRG 420
VKVEVTHRGN MRRKYRISGL TAVATRELTF PVDERNTQKS VVEYFHETYG FRIQHTQLPC 480
LQVGNSNRPN YLPMEVCKIV EGQRYSKRLN ERQITALLKV TCQRPIDREK DILQTVQLND 540
YAKDNYAQEF GIKISTSLAS VEARILPPPW LKYHESGREG TCLPQVGQWN MMNKKMINGG 600
TVNNWICINF SRQVQDNLAR TFCQELAQMC YVSGMAFNPE PVLPPVSARP EQVEKVLKTR 660
YHDATSKLSQ GKEIDLLIVI LPDNNGSLYG DLKRICETEL GIVSQCCLTK HVFKMSKQYM 720
ANVALKINVK VGGRNTVLVD ALSRRIPLVS DRPTIIFGAD VTHPHPGEDS SPSIAAVVAS 780
QDWPEITKYA GLVCAQAHRQ ELIQDLFKEW KDPQKGVVTG GMIKELLIAF RRSTGHKPLR 840
IIFYRDGVSE GQFYQVLLYE LDAIRKACAS LEAGYQPPVT FVVVQKRHHT RLFAQNHNDR 900
HSVDRSGNIL PGTVVDSKIC HPTEFDFYLC SHAGIQGTSR PAHYHVLWDE NNFTADGLQS 960
LTNNLCYTYA RCTRSVSIVP PAYYAHLAAF RARFYMEPET SDSGSMASGS MARGGGMAGR 1020
STRGPNVNAA VRPLPALKEN VKRVMFYC 1048 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:TAIR.
 GO:0019898; C:extrinsic to membrane; IDA:TAIR.
 GO:0005634; C:nucleus; IDA:TAIR.
 GO:0030529; C:ribonucleoprotein complex; IEA:UniProtKB-KW.
 GO:0004521; F:endoribonuclease activity; IDA:TAIR.
 GO:0035198; F:miRNA binding; IPI:TAIR.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0035197; F:siRNA binding; IPI:TAIR.
 GO:0009955; P:adaxial/abaxial pattern specification; IMP:TAIR.
 GO:0048830; P:adventitious root development; IMP:TAIR.
 GO:0009850; P:auxin metabolic process; IGI:TAIR.
 GO:0006952; P:defense response; IEA:UniProtKB-KW.
 GO:0009793; P:embryo development ending in seed dormancy; IGI:TAIR.
 GO:0035195; P:gene silencing by miRNA; IEP:TAIR.
 GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
 GO:0009965; P:leaf morphogenesis; IMP:TAIR.
 GO:0010589; P:leaf proximal/distal pattern formation; IMP:TAIR.
 GO:0010305; P:leaf vascular tissue pattern formation; IMP:TAIR.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
 GO:0009733; P:response to auxin stimulus; IGI:TAIR.
 GO:0010218; P:response to far red light; IGI:TAIR.
 GO:0016246; P:RNA interference; IMP:TAIR.
 GO:0048864; P:stem cell development; IGI:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0009616; P:virus induced gene silencing; IDA:TAIR.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR024357; Argonaut_Gly-rich.
 IPR014811; DUF1785.
 IPR003100; PAZ.
 IPR003165; Piwi.
 IPR012337; RNaseH-like_dom. 
Pfam
 PF08699; DUF1785
 PF12764; Gly-rich_Ago1
 PF02170; PAZ
 PF02171; Piwi 
SMART
 SM00949; PAZ
 SM00950; Piwi 
PROSITE
 PS50821; PAZ
 PS50822; PIWI 
PRINTS