| Tag | Content |
|---|
| CPLM ID | CPLM-014916 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Alpha-2-macroglobulin-P |
Protein Synonyms/Alias | Alpha-2-macroglobulin |
Gene Name | A2mp |
Gene Synonyms/Alias | A2m |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 1448 | DLKPAIVKVYDYYEK | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). |
Sequence Annotation | REGION 623 752 Bait region (By similarity).
CARBOHYD 62 62 N-linked (GlcNAc...) (Potential).
CARBOHYD 77 77 N-linked (GlcNAc...) (Potential).
CARBOHYD 253 253 N-linked (GlcNAc...) (Potential).
CARBOHYD 402 402 N-linked (GlcNAc...) (Potential).
CARBOHYD 654 654 N-linked (GlcNAc...) (Potential).
CARBOHYD 774 774 N-linked (GlcNAc...) (Potential).
CARBOHYD 869 869 N-linked (GlcNAc...) (Potential).
CARBOHYD 991 991 N-linked (GlcNAc...) (Potential).
CARBOHYD 1366 1366 N-linked (GlcNAc...) (Potential).
DISULFID 257 305 By similarity.
DISULFID 275 293 By similarity.
DISULFID 284 284 Interchain (with C-437) (By similarity).
DISULFID 437 437 Interchain (with C-284) (By similarity).
DISULFID 476 569 By similarity.
DISULFID 601 771 By similarity.
DISULFID 650 697 By similarity.
DISULFID 821 849 By similarity.
DISULFID 847 883 By similarity.
DISULFID 921 1321 By similarity.
DISULFID 1079 1127 By similarity.
DISULFID 1352 1467 By similarity.
CROSSLNK 972 975 Isoglutamyl cysteine thioester (Cys-Gln) |
Keyword | Bait region; Complete proteome; Disulfide bond; Glycoprotein; Pregnancy; Protease inhibitor; Reference proteome; Secreted; Serine protease inhibitor; Signal; Thioester bond. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1474 AA |
Protein Sequence | MGKRWLPSLA LLPLPPPLLL LLLLLLPTNA SAPQKPIYMV MVPSLLHAGT PEKGCLLFNH 60
LNETVTVKVS MESVRGNQSL FTDLVVDKDL FHCASFIVPQ SSSNEVMFLT VQVKGPTHEF 120
RRRSTVLIKT KESLVFAQTD KPIYKPGQMV RFRVVSLDEN FHPLNELIPL LYIQDSKKNR 180
IAQWQNFRLE GGLKQLSFPL SSEPTQGSYK VVIRTESGRT VEHPFSVKEF VLPKFEVKVA 240
VPETITILEE EMNVSVCGIY TYGKPVPGHV TVNICRKYSN PSSCFGEESL AFCEKFSQQL 300
DGRGCFSQLV KTKSFQLKRQ EYEMQLDVNA KIQEEGTGVE ETGKGLTKIT RTITKLSFVN 360
VDTHFRQGIP FVGQVLLVDG RGTPIPYEMI FIGADEANQN INTTTDKNGL ARFSINTDDI 420
MGTSLTVRAK YKDSNVCYGF RWLTEENVEA WHTANAVFSP SRSFVHLESL PYKLRCEQTL 480
AVQAHYILND EAVLERKELV FYYLMMAKGG IVRAGTHVLP VTQGHKKGHF SILISMETDL 540
APVARLVLYT ILPNGEVVGD TVKYEIEKCL ANKVDLVFHP NIGLPATRAF LSVMASPQSL 600
CGLRAVDQSV LLTKPEAELS ASLVYDLLPV KDLTGFPKGV NQQEEDTNGC LKQNDTYING 660
ILYSPVQNTN EEDMYGFLKD MGLKVFTNLN IRKPKVCERL GVNKIPAAYH LVSQGHMDAF 720
LESSESPTET TRSYFPETWI WDLVIVDSTG VAEMEVTVPD TITEWKAGAF CLSNDTGLGL 780
SPVIDFQAFQ PFFVDLTMPY SVIRGEAFTL KATVLNYLQT CIRVGVQLEA SPDFLATPEE 840
KEQKSHCICM NERHTMSWAV IPKSLGNVNF TVSAEALDSK ELCRNEVPVV PERGKKDTII 900
KSLLVEPEGL ENEVTFNSLL CPTGAEVSEQ ISLKLPSDVV EESARASVTV LGDILGSAMQ 960
NTQDLLKMPY GCGEQNMVLF APNIYVLDYL NETEQLTQEI KTKAITYLNT GYQRQLNYKH 1020
RDGSYSTFGD KPGRSHANTW LTAFVLKSFA QARRYIFIDE SHITQALTWL SQQQKDNGCF 1080
RSSGSLLNNA MKGGVEDEVT LSAYITIALL EMSLPVTHPV VRNALFCLDT AWKSARRGAS 1140
GNHVYTKALL AYAFALAGNQ DTKKEILKSL DEEAVKEDNS VHWTRAQKPR VPADLWYQPQ 1200
APSAEVEMTA YVLLAYLTTE LVPTREDLTA AMLIVKWLTK QQNSHGGFSS TQDTVVALHA 1260
LSKYGAATFT RAKKAAHVTI QSSGAFYTKF QVNNDNQLLL QRVTLPTVPG DYTAKVAGEG 1320
CVYLQTSLKY SVLPREKEFP FALVVQTLPG TCEDLKAHTT FQISLNISYI GSRSDSNMAI 1380
ADVKMVSGFI PLKPTVKMLE RSVHVSRTEV SNNHVLIYLD KVSNQMLTLF FMVQQDIPVR 1440
DLKPAIVKVY DYYEKDEFAV AKYSAPCSAG YGNA 1474 |
Gene Ontology | GO:0005615; C:extracellular space; IEA:InterPro. GO:0070062; C:extracellular vesicular exosome; IEA:Compara. GO:0030414; F:peptidase inhibitor activity; TAS:MGI. GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW. GO:0007565; P:female pregnancy; IEA:UniProtKB-KW. GO:0001869; P:negative regulation of complement activation, lectin pathway; IEA:Compara. GO:0010951; P:negative regulation of endopeptidase activity; IEA:GOC. GO:0048863; P:stem cell differentiation; IDA:MGI. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |