CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024310
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cullin-4B 
Protein Synonyms/Alias
 CUL-4B 
Gene Name
 Cul4b 
Gene Synonyms/Alias
 mKIAA0695 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
315KISANLYKQLRQICEubiquitination[1]
665DVFEAFYKKDLAKRLubiquitination[1]
676AKRLLVGKSASVDAEubiquitination[1]
691KSMLSKLKHECGAAFacetylation[2]
707SKLEGMFKDMELSKDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation- induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (By similarity). 
Sequence Annotation
 MOTIF 112 115 Nuclear localization signal (By
 MOD_RES 106 106 Phosphothreonine (By similarity).
 MOD_RES 110 110 Phosphoserine (By similarity).
 MOD_RES 200 200 Phosphoserine (By similarity).
 MOD_RES 250 250 Phosphoserine (By similarity).
 CROSSLNK 916 916 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Cell cycle; Complete proteome; DNA damage; DNA repair; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 970 AA 
Protein Sequence
MSRSTRSKER RENDTDSEDN SSETSNQERR RCRQGPPRPP YPPLLPPVFP PPTPPPQVRR 60
TRGLQDLGAM KSVCPGTSGF SSPNPSAASA AAQEVRSATD GNTSTTPPTS AKKRKLNSSS 120
SSSNSSNERE DFDSTSSSST PPQPRDSASP STSSFCLGVP VATSSHVPIQ KKLRFEDTLE 180
FVGIDTKMAE ESSSSSSSSS PTAATSQQQQ QQQLKTKSIL ISSVASVHHA NGLAKSSTAV 240
SSFANSKPGS AKKLVIKNFK DKPKLPENYT DETWQKLKEA VEAIQNSTSI KYNLEELYQA 300
VENLCSHKIS ANLYKQLRQI CEDHIKAQIH QFREDSLDSV LFLKKIDRCW QNHCRQMIMI 360
RSIFLFLDRT YVLQNSMLPS IWDMGLELFR AHIISDQKVQ TKTIDGILLL IERERNGEAI 420
DRSLLRSLLS MLSDLQIYQD SFEQQFLQET NRLYAAEGQK LMQEREVPEY LHHVNKRLEE 480
EADRLITYLD QTTQKSLIAS VEKQLLGEHL TAILQKGLNS LLDENRIQDL SLLYQLFSRV 540
RGGVQVLLQQ WIEYIKAFGS TIVINPEKDK TMVQELLDFK DKVDHIIDTC FLKNEKFINA 600
MKEAFETFIN KRPNKPAELI AKYVDSKLRA GNKEATDEEL EKMLDKIMII FRFIYGKDVF 660
EAFYKKDLAK RLLVGKSASV DAEKSMLSKL KHECGAAFTS KLEGMFKDME LSKDIMIQFK 720
QYMQNQNVPG NIELTVNILT MGYWPTYVPM EVHLPPEMVK LQEIFKTFYL GKHSGRKLQW 780
QSTLGHCVLK AEFKEGKKEL QVSLFQTMVL LMFNEGEEFS LEEIKHATGI EDGELRRTLQ 840
SLACGKARVL AKNPKGKDIE DGDKFICNDD FKHKLFRIKI NQIQMKETVE EQASTTERVF 900
QDRQYQIDAA IVRIMKMRKT LSHNLLVSEV YNQLKFPVKP ADLKKRIESL IDRDYMERDK 960
ENPNQYNYIA 970 
Gene Ontology
 GO:0031465; C:Cul4B-RING ubiquitin ligase complex; ISS:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:1900087; P:positive regulation of G1/S transition of mitotic cell cycle; IMP:MGI.
 GO:0045732; P:positive regulation of protein catabolic process; IMP:MGI.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR016157; Cullin_CS.
 IPR016158; Cullin_homology.
 IPR001373; Cullin_N.
 IPR019559; Cullin_neddylation_domain.
 IPR016159; Cullin_repeat-like_dom.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00888; Cullin
 PF10557; Cullin_Nedd8 
SMART
 SM00182; CULLIN
 SM00884; Cullin_Nedd8 
PROSITE
 PS01256; CULLIN_1
 PS50069; CULLIN_2 
PRINTS